Re: Hi/low profile blades
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From: | DayDawning@aol.com |
To: | jim@proscitech.com.au, PHOBOS11@aol.com, histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Mon, 11 Oct 1999 08:20:41 EDT |
Content-Type: | text/plain; charset="us-ascii" |
It is my opinion that a good holder is most certainly a key issue when
choosing a microtome blade. Many histotechs that still use and prefer
standard blades have not had the advantage to try a good microtome knife
base. A blade holder inserted in what is designed for a standard blade
gives more chance for vibration as opposed to a knife holder that is intended
to be used with a disposable blade. Keep this in mind when making your
selection.
Dawn M. Truscott
Zeiss/Microm
In a message dated 10/10/1999 9:51:27 AM Eastern Daylight Time,
jim@proscitech.com.au writes:
<< Assuming equally good holders, a low profile blade, is more likely to
shudder
or otherwise move when challenged by a tough specimen, than is a high
profile
knife. Not surprisingly a lot of people chose thicker, high profiles blades
for
cryostat sectioning and thinner low profile blades for sectioning at 3 or
less
micrometers. >>
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