Re: Hi/low profile blades

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From:DayDawning@aol.com
To:jim@proscitech.com.au, PHOBOS11@aol.com, histonet@pathology.swmed.edu
Reply-To:
Date:Mon, 11 Oct 1999 08:20:41 EDT
Content-Type:text/plain; charset="us-ascii"

It is my opinion that a good holder is most certainly a key issue when 
choosing a microtome blade.  Many histotechs that still use and prefer 
standard blades have not had the advantage to try a good microtome knife 
base.    A blade holder inserted in what is designed for a standard blade 
gives more chance for vibration as opposed to a knife holder that is intended 
to be used with a disposable blade.  Keep this in mind when making your 
selection.
Dawn M. Truscott
Zeiss/Microm

In a message dated 10/10/1999 9:51:27 AM Eastern Daylight Time, 
jim@proscitech.com.au writes:

<< Assuming equally good holders, a low profile blade, is more likely to 
shudder 
 or otherwise move when challenged by a tough specimen, than is a high 
profile 
 knife. Not surprisingly a lot of people chose thicker, high profiles blades 
for 
 cryostat sectioning and thinner low profile blades for sectioning at 3 or 
less 
 micrometers. >>



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