Re: GI's and processor problems

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To:"Jennings-Siena, Debbie" <>
Date:Fri, 1 Oct 1999 08:57:05 -0400
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It sounds at though the biopsies are over-processed and cooked.  I don't know if
retreiving nuclear detail is possible at this point.

I have two suggestions:

Try requenching the tissue by placing the blocks that have been faced-off on a
try with ice saturated in water and let them soak until they appear swollen.
I can send you an EM Science product called Mollifex that will soften the tissue
and maybe help regain some of the nuclear detail.  No obligation.  Just send me
your address.

Rande Kline, HT (ASCP)
Technical Services
EM Science

"Jennings-Siena, Debbie" <> on 09/30/99
12:52:53 PM

To:   "''" <>
cc:    (bcc: Rande Kline/EMI/Merck)
Subject:  GI's and processor problems

Please help me figure this out.  We had a processor problem last night.  Our
rotary open style processor messed up last night and all of the GI biopsies
got dunked in each solution twice.  I pryed them out of the paraffin coated
basket and then made new cassettes.  Removed the tissues and heated them for
10 minutes and then dunked them in 2 changes of xylene and 1 change of
absolute for 5 minutes each.  Then we reprocessed starting in 95% alcohol,
100% alcohol and then xylene and paraffin.  Today, the sections are
unreadable according to the pathologists.  There is no nuclear detail and
the tissues are very eosinophilic.  I am racking my brain trying to figure
this out.  I decided that maybe some one with a clear head could help out.
We have had our slide stainer, our processor and our coverslipper all break
this week.  So I am just trying to put out fires everywhere. Thanks for your

Debbie Jennings-Siena
Baylor University Medical Center

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