Re: Fixation before decalcification
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Simon Smith <SimonS@skeletech.com> |
Reply-To: | |
Date: | Wed, 13 Oct 1999 14:01:58 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Wed, 13 Oct 1999, Simon Smith wrote:
> Anybody have first hand experience with demineralization of tissues fixed in
> NBF versus 70% alcohol?
70% alcohol would not be a good fixative for animal tissues.
I have had lots of experience of formic acid decalcification
of animal heads that were fixed in an alcohol-formalin-acetic acid
mixture. Results were fine. I think your tissue needs to be
well fixed before decalcifying, but the identity of the actual
fixative does not matter much. Choose the fixative most
appropriate to the intended embedment, staining, histochemistry
etc.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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