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-----Original Message-----
From: HistoNet Server <histonet@pathology.swmed.edu>
To: HistoNet Server <histonet@pathology.swmed.edu>
Date: Wednesday, October 13, 1999 1:22 AM
Subject: Daily Digest


>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 02:31:04 -0500
>From: "Sarah A. Jones" <hawkmoon15@earthlink.net>
>Subject: Marking ink interfering with IHC for ER/PR studies
>
>Nigel & all of Histonet-land,
>
>It is my understanding (and also the disclaimer statement made on the
>bottles of Davidson inks), that these inks CAN interfere with ER/PR
studies.
>
>The question I have is that, if we KNOW these inks CAN interfere with ER/PR
>studies, then how might they effect the other antibodies in their
>performance?  Does anyone out there have any experience that might shed
some
>light on this question?
>
>Looking forward to your usual great responses,
>Sarah A. Jones
>
><hawkmoon15@earthlink.net>
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 04:46:10 -0500
>From: "Alan Bright" <Bright@dial.pipex.com>
>Subject: Re: DisposableMicrotomeBlades
>
>Dear Neil,
>You could try to put a new edge on your IEC anti-roll plate by placing it
>vertically onto a 1000 grit piece of wet & dry paper, placed onto a flat
>surface. Move the anti-roll plate on its length, and make sure that you
hold
>it firmly to achieve a straight edge. This will renew the damaged area.
>
>Another alternative is to purchase from:-
>
>Hacker Instruments Inc.
>17 Sherwood Lane
>Fairfield
>New Jersey 07004
>USA
>
>Tel. (973) 226-8450 or 1-800-4-HACKER
>Fax. (973) 808-8281
>e-mail: HACKERLAB@AOL.COM
>
>a Magna Plate Anti-Roll Plate, Part No. 50300-1, this will fit all
cryostats
>and is placed directly onto the knife or blade without requiring any
setting
>up, it does not need to use any of the positioning mechanism fitted to the
>cryomicrotome microtome. Hacker Instruments  will also be able to supply a
>disposable blade system too.
>
>Best Regards
>
>Alan Bright
>
>Bright Instrument Co.Ltd.
>St Margarets Way
>Huntingdon
>PE18 6EB
>England
>
>Tel No:+44 (0)1480 454528
>Fax No:+44 (0)1480 456031
>Email: AlanBright@brightinstruments.com
>Web Site: www.brightinstruments.com
>
> ++++++++++++++++++++++++++++++++++++++++++==
>- -----Original Message-----
>From: Neal Beeman <neal.beeman@uchsc.edu>
>To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
>Date: Tuesday, October 12, 1999 12:02
>Subject: DisposableMicrotomeBlades
>
>
>>I have been cutting frozen sections on a Minotome model cryostat from
>>Damon/IEC.  It holds a basic Reichert microtome knife.  The microtome is a
>>multiuser instrument and the knifes get beat up as fast as they can be
>>sharpened.  An even bigger problem is that the plastic antiroll plate has
>>become ragged and does almost as much harm as good.  I want to go to a
>>disposable blade system with a nice shiny new anti roll device.  Any
>>suggestions?
>>
>>Neal
>>
>>
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 06:45:52 -0500
>From: Marcia Bentz <mb7x@virginia.edu>
>Subject: Re: Saponin reference
>
>Hi Patti,
>This is the reference I have:
>"Cytokine producing cells". eds D. Fradelizie & D. Emelie INSERM, Paris. In
>press 1994. My copy was copied and faxed, so I'm not sure of the spelling.
>The article is by Ulf Andersson and Jan Andersson. Article title:
>Immunolabeling of cytokine producing cells in tissues and in suspension.
>
>Hope this helps, even if it's not exact.
>
>Marcia
>
>At 09:57 AM 10/11/1999 -0400, Bourassa, Patricia wrote:
>>Hi, all!
>>
>>I can't seem to find my paper on use of Saponin in IHC.  Does anyone out
>>there have a good reference they use?  I'm hoping it matches mine!
>>
>>Thanks!
>>
>>-Patti Bourassa
>> Pfizer, Groton
>>
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 07:01:44 -0500
>From: Rod Slyter <rlslyter@hsc.vcu.edu>
>Subject: Re: Bone Saw
>
>Patty,
>If the majority of the bones you need to cut are femoral heads. A simple
and
>cheap fix is to get a mallet and old dull large knife. You can easily split
>the
>bone in two with a couple god wacks an the back to the blade with mallet.
No
>mess, no bone dust from the saw. You then throw the bone in 1/2 decal and
1/2
>formalin until you can cut off you section with a scalpel.
>
>Rod Slyter, PA (AAPA)
>
>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 08:01:01 -0500
>From: Jill Songer <jtsonger@vt.edu>
>Subject: RE: Tissue Slicer
>
>Sarah,
>We got our tissue slicer from Thomas Scientific 1-800-345-2100.
>
>**************************************************************************
>Jill Songer HT (ASCP)
>Virginia-Maryland Regional College of Veterinary Medicine
>Veterinary Teaching Hospital
>Virginia Tech
>
>"To achieve the possible, we must attempt the impossible"
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 08:32:52 -0500
>From: rschoonh@sph.unc.edu
>Subject: Re: Hi/low profile blades
>
>I totally agree with Alan Bright on this.   Having been involved with the
>manufacturing, QA, QC and development of what were known as the AO/Reichert
>and
>Reichert Jung disposable microtome blades (in a past industry life), I can
>attest that manufacturing a disposable blade specifically for cryostat use
>could
>be cost prohibitive.  The entire production line would have to be switched
>over
>to using a low temperature oil which would also have to be able to act as a
>lubricant at high temperatures.   This oil would also have to not react
with
>the
>polymers used to coat the blade.  Not an easy or cheap thing to do.  I
know,
>as
>I did think of this while we were developing the blades.  It must be noted
>that
>the oil coating on the blades is not just there to make dispensing the
blade
>easier but primarily for the actual production of the blade.
>
>I would note that it will take longer (in most cases)for the OCT to freeze
>than
>for the disposable blade to come to cryostat temperature.
>
>best regards,
>Bob
>Robert Schoonhoven
>Laboratory of Molecular Carcinogenesis and Mutagenesis
>Dept. of Environmental Sciences and Engineering
>University of North Carolina
>CB#7400
>Chapel Hill, NC 27599
>Phone
>office 919-966-6343
>   Lab 919-966-6140
>   Fax 919-966-6123
>
>Don't go around saying the world owes you a living; the world owes you
>nothing; it was here first.
>Mark Twain [Samuel Langhornne Clemens] (1835-1910)
>
>- -- Begin original message --
>
>> From: Alan Bright <Bright@dial.pipex.com>
>> Date: Mon, 11 Oct 1999 14:51:33 +0100
>> Subject: Re: Hi/low profile blades
>> To: DayDawning@aol.com, jim@proscitech.com.au, PHOBOS11@aol.com,
>>  histonet@pathology.swmed.edu
>>
>> Dear Dawn,
>>
>> I cannot see the point of keeping spare disposable blades in the
>> cryochamber, as when they are placed into the blade holder they take less
>> than 30 secs. to reach sectioning temperature. Plus the fact as a pack of
>> blades are already very expensive, and to have this modified for cryowork
>> which would be very simple, would only give an excuse to charge more.
>>
>> Best Regards
>>
>> Alan Bright
>>
>> Bright Instrument Co.Ltd.
>> St Margarets Way
>> Huntingdon
>> PE18 6EB
>> England
>>
>> Tel No:+44 (0)1480 454528
>> Fax No:+44 (0)1480 456031
>> Email: AlanBright@brightinstruments.com
>> Web Site: www.brightinstruments.com
>>
>>
>>
>>
>> -----Original Message-----
>> From: DayDawning@aol.com <DayDawning@aol.com>
>> To: jim@proscitech.com.au <jim@proscitech.com.au>; PHOBOS11@aol.com
>> <PHOBOS11@aol.com>; histonet@pathology.swmed.edu
>> <histonet@pathology.swmed.edu>
>> Date: Monday, October 11, 1999 01:35
>> Subject: Re: Hi/low profile blades
>>
>>
>> >In a message dated 10/10/1999 9:51:27 AM Eastern Daylight Time,
>> >jim@proscitech.com.au writes:
>> >
>> ><<  Not surprisingly a lot of people chose thicker, high profiles blades
>> for
>> > cryostat sectioning and thinner low profile blades for sectioning at 3
or
>> >less
>> > micrometers. >>
>> >And another thing....
>> >Why doesnt someone make a blade that is in a cryo-proof dispenser?  The
>> >blades have a fine coating of oil and if left in the cryostat (like a
good
>> >tech should, always prepared with an extra blade, ) the oil  freezes and
is
>> >hard to get out of the box.
>> >Dawn Truscott
>> >Zeiss/Microm
>> >
>>
>>
>>
>
>- -- End original message --
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 09:01:00 -0500
>From: "Lori Donnelly" <LDonnelly@genetics.com>
>Subject: Looking for Roslyn Feeley
>
>I am curious as to whether anyone knows the whereabouts of Roslyn Feeley,
who
>used to work at Millennium Pharmaceuticals, Inc., in Cambridge, MA.  If you
>have any contact information that you can give me, please contact me
>personally at ldonnelly@genetics.com or (978) 247-1336.  Thanks in advance
for
>any help you can provide.
>
>Lori Hayes Donnelly
>Genetics Institute, Inc.
>Andover, MA
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 09:23:34 -0500
>From: Marcia Bentz <mb7x@virginia.edu>
>Subject: source of IgG isotype controls
>
>Hi,
>My IHC staining is going well, but I need to find Rat IgG and Goat IgG
>isotype controls to make sure my staining is specific. I've looked through
>Dako, Pharmingen, Caltag, Vector. My primaries are Rat anti-mouse, and Goat
>anti-mouse.
>Any help in locating these would be great!
>Thanks,
>Marcia
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 09:24:01 -0500
>From: RSRICHMOND@aol.com
>Subject: Bone Saw
>
>Patty Eneff in Oklahoma City asks about bone saws, noting that
>
>>>The pathologist swears that a [scroll] saw was used at Johns Hopkins,
where
>he did his residency.<<
>
>Last year I was at Johns Hopkins for a continuing medical education program
>that included a talk by a pathologist there who is interested in the
>pathology of femoral heads and such. Unlike your pathologist, I took notes
>(something every pathology resident should do about methods used in their
>residency - you're likely to need them in your first jobs, as your
>pathologist found out) and did some comparison shopping at Home Depot. My
>notes read:
>
>Edward F. McCarthy, Jr., M.D. at Johns Hopkins uses a table scroll saw from
>Home Depot, used by woodworkers to cut out fancy woodwork - a vibrating
saw,
>you press the bone down into the sawis table to cut. [I checked these right
>after the conference in May 1998 - about $90 for a saw of mainland Chinese
>manufacture, about $180 for a saw made in Taiwan. The saws are heavy, with
a
>large footprint, about 18 inches on a side, probably too large for most
gross
>rooms.] Take the dust off the sawn surface by gently brushing with a
>toothbrush.
>
>Bob Richmond
>Samurai Pathologist
>Knoxville TN
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 13:30:32 -0500
>From: "Baileyhealy, Irene {Immu~Palo Alto}" <IRENE.BAILEYHEALY@ROCHE.COM>
>Subject: RE: source of IgG isotype controls
>
>Hi Marcia
>I buy mine from Zymed. 1-800-874-4494 or www.zymed.com
>
>Irene
>
>
>
>Irene Bailey-Healy
>Biologist
>Roche Bioscience
>
>> -----Original Message-----
>> From: Marcia Bentz [SMTP:mb7x@virginia.edu]
>> Sent: Tuesday, October 12, 1999 7:00 AM
>> To: histonet@pathology.swmed.edu
>> Subject: source of IgG isotype controls
>>
>> Hi,
>> My IHC staining is going well, but I need to find Rat IgG and Goat IgG
>> isotype controls to make sure my staining is specific. I've looked
through
>> Dako, Pharmingen, Caltag, Vector. My primaries are Rat anti-mouse, and
>> Goat
>> anti-mouse.
>> Any help in locating these would be great!
>> Thanks,
>> Marcia
>>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 14:31:17 -0500
>From: Mary Lou Norman <mlm11@cornell.edu>
>Subject: Re: Statie Riggs tissue slicer
>
>Sarah,
>Though I have never used it, seems like I've acquired one.  Arthur H.Thomas
>Co.  is on the box.
>
>Mary Lou
>
>At 15:05 1999-10-11 -0500, you wrote:
>>Hello Netters,
>>  Had a researcher ask about a Statie Riggs tissue slicer.  Has anyone
>heard of these and where they can be purchased?  Thanks, Sarah
>>
>>Sarah Christo, HT (ASCP)
>>Texas A&M University
>>College of Veterinary Medicine
>>Dept. of Vet. Anatomy & Public Health
>>Histology Laboratory
>>College Station, TX  77868-4458
>>schristo@cvm.tamu.edu
>>phone (409) 845-3177
>>fax (409) 847-8981
>>
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 15:11:11 -0500
>From: Patricia Karlisch <PKARLISCH@PSGHS.EDU>
>Subject: TIMEMED
>
>Hi,
>  Does anyone out there have the address and phone number of the Label
>company called TIMEMED in Illinois.  I would like to have custom made
labels
>and they always did a great job.
>Thanks, Pat Karlisch
>Geisinger Medical Center
>Tel: 570-271-8148
>Fax:  570-271-5916
>Email:  pkarlisch@psghs.edu
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 15:11:36 -0500
>From: Colleen Forster <cforster@tc.umn.edu>
>Subject: Cox 2 staining in mouse tissue
>
>Hello histonetters,
>
>Again the question on COX 2 comes  up however, I am interested in an
>antibody that has worked in mouse tissue, specifically brain. Any help
>would be appreciated.
>
>Colleen Forster
>U of MN
>Dept. of Neurology
>Ph 612 626-2477
>Fax 612 625-7950
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 15:12:25 -0500
>From: Jill Songer <jtsonger@vt.edu>
>Subject: cells from culture
>
>Hi Histonetters,
>
>Before everyone, except me, heads off to NSH, let me pick your brains. We
>have a researcher who has brain cells grown from cell cultures. These cells
>are in agar. Sorry, don't know what kind. He is interested in doing
>immunocytochemistry on the cells and wonders if the agar will interfere
>with the immuno label. Again, I don't know what label. Also, any tips on
>processing these little suckers and how to embed them without losing them.
>Will the agar stay put during processing or will it dissolve?
>
>Thanks.
>
>**************************************************************************
>Jill Songer HT (ASCP)
>Virginia-Maryland Regional College of Veterinary Medicine
>Veterinary Teaching Hospital
>Virginia Tech
>
>"To achieve the possible, we must attempt the impossible"
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 16:38:23 -0500
>From: "Goodwin, Diana" <DGoodwin@CHSNJ.org>
>Subject: Histonet Button
>
>Hello, Netters.
>
>I will not be able to attend the NSH Symposium this year.  Is it
>possible for me to get a Histonet button without being in attendance?
>
>
>Diana Goodwin,  HT
>Trenton,  NJ
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 16:42:36 -0500
>From: neal.beeman@uchsc.edu (Neal Beeman)
>Subject: DisposableMicrotomeBlades
>
>I have been cutting frozen sections on a Minotome model cryostat from
>Damon/IEC.  It holds a basic Reichert microtome knife.  The microtome is a
>multiuser instrument and the knifes get beat up as fast as they can be
>sharpened.  An even bigger problem is that the plastic antiroll plate has
>become ragged and does almost as much harm as good.  I want to go to a
>disposable blade system with a nice shiny new anti roll device.  Any
>suggestions?
>
>Neal
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 16:43:22 -0500
>From: neal.beeman@uchsc.edu (Neal Beeman)
>Subject: GFP/IHC unclear
>
>Thanks to everyone replying to my 10/7 question.  I was not clear in
>stating my question.
>        I am developing a virus based gene therapy/gene delivery model for
>the mammary epithelium.  I have been successful in demonstrating the innate
>fluorescent signal from the green fluorescent protein reporter gene product
>(the GFP).  I want to be able to see the innate fluorescent signal from the
>green fluorescent reporter gene product in transduced cells and to
>simultaneously be able to see milk proteins in these same cells using
>immunofluorescence techniques long established in my lab.  Specifically,
>can the innate fluorescence from the GFP withstand the detergent and NaBH4
>that I have been using in the immunofluorescent detection of milk proteins?
>
>
>Neal
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 17:45:14 -0500
>From: "MacDonald, Jennifer" <jmacdonald@sach.org>
>Subject: RE: Statie Riggs tissue slicer
>
>What is a Statie Riggs tissue slicer??
>
>
>> Mary Lou
>>
>> At 15:05 1999-10-11 -0500, you wrote:
>> >Hello Netters,
>> >  Had a researcher ask about a Statie Riggs tissue slicer.  Has anyone
>> heard of these and where they can be purchased?  Thanks, Sarah
>> >
>> >Sarah Christo, HT (ASCP)
>> >Texas A&M University
>> >College of Veterinary Medicine
>> >Dept. of Vet. Anatomy & Public Health
>> >Histology Laboratory
>> >College Station, TX  77868-4458
>> >schristo@cvm.tamu.edu
>> >phone (409) 845-3177
>> >fax (409) 847-8981
>> >
>> >
>> >
>>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 17:45:49 -0500
>From: Colleen Forster <cforster@tc.umn.edu>
>Subject: 4% para vs 10% bnf
>
>Histonetters,
>
>I have an educational test question I need help with. What is the
>difference in how para works compared to BNF ??
>
>I use 4% para for all my research profusion and wonder why that is
>recommended over 10% BNF? Also what is the actual chemistry going on
>that para needs to be at 4 C when fixing vs room temp like BNF??/
>
>Anyone know the answers to the tivia I would appreciate it.
>
>
>Colleen Forster
>U of MN
>Dept. of Neurology
>Ph 612 626-2477
>Fax 612 625-7950
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 18:26:58 -0500
>From: Ann Thornton <pathat@wnmeds.ac.nz>
>Subject: M30 antibody
>
>Is anyone using the Boehringer M30 CytoDEATH antibody for the detection of
>apoptosis??  I have tried various dilutions but have only seen positive
>nuclear staining.  Additional dilutions have produced fewer positive
>nuclear staining, but none of the granular cytoplasmic staining expected.
>
>Any help would be appreciated.
>
>Ann
>Ann Thornton
>Department of Pathology
>Wellington School of Medicine
>Wellington
>New Zealand
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 18:50:24 -0500
>From: Cathy Mayton <Cathy@wasatchhisto.com>
>Subject: blades for isomet
>
>Fellow histonetters,
>
>This may be helpful to owners of Isomet saws.  There are several sources
>for diamond cut-off blades for the Isomet.  I have purchased my blades from
>a lapidary store (rock shop) in Arizona for years now.  The blades are
>extremely reasonable in price (about $25.00) and they they do not chip out
>like some other brands do.  There are 2 other sources that I am aware of,
>but because I order a fair amount of blades I can save a few more dollars
>by purchasing them from Rockazona.  Even if your residents are rough on the
>blades, for the money you could buy many of these blades for 1 Buehler
blade.
>
>If, interested drop me an email.  See ya in Rhode Island!!
>
>Regards,
>Cathy
>
>
>
>***************************************
>Cathy A. Mayton
>Wasatch Histo Consultants, Inc.
>80 Youngberg Road
>Winnemucca, NV   89445-5709
>Voice:  (775) 625-4425
>Fax: (775) 625-1548
>www.wasatchhisto.com
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 19:07:54 -0500
>From: barbwebb@webtv.net (barbara webb)
>Subject: Re: Histonet Button
>
>Diana-
>I'll  be at  NSH  in  Providence  and
>would  be glad to get one  for you or
>if  I  only  get one to send it  to you
>for  your collection  - I  haven't  been
>to  National  for a few  years  -do you
>know  how  they are  generally given
>out?
>B Webb HTL(ASCP)
>on the beautiiful
>Eastern Shore of Maryland
>
>
>
>----------------------------------------------------------------------
>
>Date: 12 Oct 1999 21:15:47 -0500
>From: "Muhammad Tahseen" <tahseen@brain.net.pk>
>Subject: CD 5
>
>My pathologist is interested in using CD 5 on paraffin section. Anyone
>have experience with such. Thanks in
>advance.
>
>Muhammad Tahseen
>Histology Supervisor,
>SKMT&RC.
>Lahore,Pakistan.
>
>
>
>
>
>Here are the messages received yesterday!
>