Re: Bouin's solution
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | marilyn.johnson@agric.gov.ab.ca |
Reply-To: | |
Date: | Thu, 7 Oct 1999 21:46:14 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Thu, 7 Oct 1999 marilyn.johnson@agric.gov.ab.ca wrote:
> Does anyone know if there is an expiry date for Bouin's solution?
Aqueous Bouin can be kept for ever - well at least 5 or 6 years
anyway. This is the picric-formalin-acetic fixative that most
people call Bouin's fluid (though he did invent more than one).
Alcoholic Bouin and similar mixtures do change with time
(months) and for some purposes the fixation is said to be
better with an aged solution. Its composition is known, and
you can make artificially aged alcoholic Bouin directly.
I can provide the details and reference if you're interested.
Chuck Churukian's "Manual of the Special Stains Laboratory"
(Rochester, NY, 1997) has a big table of shelf lives. I don't
have my copy to hand right now, but would guess that he says
6 months. Many of the times in his table seem far too short;
there are even shelf lives for solutions that would last for
centuries.
You need to know of the potential explosive hazard
of dry picric acid. If there's some dry yellow stuff encrusted
round the stopper or cap of the bottle, run it under the tap
to make it wet. Picric acid explodes when its temperature
gets above 300C. This seems unlikely from the friction of
undoing a tight stopper and is, of course, impossible in the
presence of liquid water. The stock jar of picric acid must
be inspected from time to time, and should always have a
generous layer of water above the powder.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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