Dear Karen,
	
	Boehringer Mannheim (USA Tel.: (8 00) 4 28 54 33) produce a free (at least
here in Aus)  book called "Nonradioactive In Situ Hybridization Application
Manual, 2nd Edn"  It has excellent methods fully referenced for this type
of application.  While I have not had to do any In Situ work, I have used
this manual for DIG methods, & found them to be excellent.

	Other texts include "Apoptosis & Cell Proliferation, 2nd Edn" & "The DIG
System User's Guide for Filter Hybridization".

	Regards

	Rob W.

At 15:16 10/04/1999 -0500, you wrote:
>I see the in situ question has arisen again.  We have still not gotten
>anywhere with this and our pathologist is trying to be patient.  He wants
>us to do in situ on pancreas slides, paraffin and formalin fixed.  We are
>using an oligo probe to insulin and tail it with DIG.
>Does anyone have a tried and true method for doing this type of in situ??
>Marianne and I don't feel qualified to do this without  molecular
>experience but seems like we have to try to learn it.
>Karen Dulany  HTL (ASCP)
>Eppley  Institute for Cancer Research
>Omaha, NE
>402-559-5123


R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
E-mail	r.wadley@unsw.edu.au
www	http://www.micro.unsw.edu.au/caf.html