Re: Bobrowitz-InSitu

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To:Dana Settembre <settembr@UMDNJ.EDU>
Date:Mon, 4 Oct 1999 15:16:36 -0500
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I see the in situ question has arisen again.  We have still not gotten
anywhere with this and our pathologist is trying to be patient.  He wants
us to do in situ on pancreas slides, paraffin and formalin fixed.  We are
using an oligo probe to insulin and tail it with DIG.

Does anyone have a tried and true method for doing this type of in situ??
Marianne and I don't feel qualified to do this without  molecular
experience but seems like we have to try to learn it.

Karen Dulany  HTL (ASCP)
Eppley  Institute for Cancer Research
Omaha, NE

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