RE: yellow counterstains (was Thomas Histology)
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From: | "Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA> |
To: | histonet@pathology.swmed.edu, "'RSRICHMOND@aol.com'" <RSRICHMOND@aol.com> |
Reply-To: | |
Date: | Thu, 07 Oct 1999 13:03:10 -0400 |
Content-Type: | text/plain |
Bob,
When I was a small boy, I was both obstreperous and wiggly!
Do you think that the very small amount of yellow#5, that was present(
mainly in orange juice and dried eggs) in a wartime diet in the UK, accounts
for my fondness for PAS counterstained with Tartrazine?
BTW I'd love to hear the yarn about Martius yellow.
Bryan
> ----------
> From: RSRICHMOND@aol.com[SMTP:RSRICHMOND@aol.com]
> Sent: October 7, 1999 8:41 AM
> To: histonet@pathology.swmed.edu
> Subject: yellow counterstains (was Thomas Histology)
>
> Hewlett Bryan notes:
>
> >>I have used saturated tartrazine in 70% cellosolve as a counterstain to
> PAS
> for over 35 years. The solution is much more stable than Metanil yellow.
> Martius yellow is an excellent alternative, as is an alcoholic solution of
>
> Picric acid. I know, I know!!! All you safety minded people stay OFF my
> back!<<
>
> Tartrazine (C.I. 19140) is the infamous F.D.C. Yellow #5, the food
> additive
> without which junk food as we know it would not be possible. This odd fact
>
> may account for the rather ominous warning labels I've sometimes seen on
> containers of tartrazine for laboratory use. It would appear to be a
> minimal
> risk laboratory chemical.
>
> It's an Article of Faith in Alternative Medicine that F.D.C. Yellow #5 is
> the
> Great Satan of junk food, or one of them at any rate, though I think the
> evidence here is fairly minimal. Perhaps the most convincing accusation -
> probably not very convincing - is that it has something to do with the
> obstreperous wiggliness of small boys.
>
> I have a yarn about Martius yellow (C.I. 10315) too, but it probably
> doesn't
> belong in this thread (arrgh!)
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
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