Nevermind.  Turns out that "new reagents" means never opened.  The reagents
were very expired, but unopened.

> -----Original Message-----
> From:	Bennett, Catherine (Katie) [SMTP:cbennett@lrri.org]
> Sent:	Friday, October 15, 1999 2:03 PM
> To:	'histonet@pathology.swmed.edu'
> Subject:	PAP Stain
> 
> Does anyone know why a PAP stain procedure would start staining cells
> orange
> which used to be staining blue - specifically Type II epithelium cells
> from
> lung epithelial cytospins?  
> 
> We are trying to figure out what has changed with our routine staining
> procedure.  Typically the Type II cells stain a nice light blue with
> purple
> granules.  Now for some reason, they are staining what is being termed as
> a
> "nice suede color" - kinda orange brown.  If you look real carefully, you
> the granules of the Type II cells are still staining purple, but the rest
> of
> the cell is orange now instead of blue.  Apparently the tech doing the
> staining has bought all new reagents and we don't know what is going wrong
> this time.  Any suggestions/advice?
> 
> *********************************
> Catherine "Katie" Bresee Bennett
> Sr. Technical Associate
> Lovelace Respiratory Research Institute
> Albuquerque, New Mexico
>