RE: Cerb (her2) staining in a bone marrow biopsy

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:'histonet' <>, 'Tom Wells' <>
Date:Thu, 14 Oct 1999 15:14:37 -0400


I have used this antibody for a number of years, on all sorts of material.
I cannot answer from experience the first question, but I suspect there is
little effect provided that the fixation is good.
B5 fixation is absolutely fine for c-erbB-2. Back in 1988 I did a study
comparing NBF and B5 fixation on breast tumors. The Dako polyclonal antibody
to c-erbB-2 was just one of the antibodies used. Very nice, crisp, membrane


> ----------
> From: 	Tom Wells[]
> Sent: 	October 13, 1999 8:14 PM
> To: 	'histonet'
> Subject: 	Cerb (her2) staining in a bone marrow biopsy
> I have a question regarding cerb (her2) staining. We are using the Dako
> polyclonal antibody to cerb and we are very pleased with the results.
> However, I was wondering if anybody has any experience with the effects of
> (1) decalcification with HCl and (2) B5 fixation on cerb staining. We want
> to stain mets in a bone marrow, unfortunately, we don't have access to the
> primary breast tissue. So we have no way of checking our results.  Any
> comments would be appreciated!  Thanks. Tom

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