May-Grunwald Stain

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From:"Nancy Maronto" <>
Date:Fri, 08 Oct 1999 05:17:26 PDT
Content-Type:text/plain; format=flowed

Hi Histonetters,
Is there anyone who's lab is staining bone marrows smears(animal species - 
rat, mouse, etc.) with Richard-Allan's May-Grunwald stain solution?  We have 
tried a few combinations of this stain: Increasing the time of the stain (10 
min) and stain/buffer (10 min), and different timings of these two steps.  
This resulted in a desirable stain for the thin areas of the smear, with too 
blue staining in the thick areas.  Technical support from Richard-Allan 
suggests changing the stain/buffer concentrations without changing timings.  
We will try this, but would like any other input from techs that may have 
this worked out.  Slides are fixed in methanol. We usually try a few 
combinations at the same time to save tech time.  Thanks in advance for your 

Nancy Maronto
Histology Special Procedures Lab
MPI Research
Mattawan, MI

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