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From:Neal Beeman <>
Date:Thu, 07 Oct 1999 20:02:27 -0700
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I want to know if anyone has a protocol for doing IHC on frozen sections
that does not destroy the signal from green fluorescent protein.  I am
transducing the mammary epithelium in mice with an adenovirus construct that
encodes GFP as a reporter gene.  I want to show that transduced cells
express endogenous milk proteins normally.  I am worried that the IHC
procedure I have been doing on mammary frozen sections for milk proteins
will destroy GFP signal.  Specifically, can GFP withstand detergent and can
GFP withstand NaBH4?  


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