GFP/IHC unclear

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From:neal.beeman@UCHSC.edu (Neal Beeman)
To:histonet@pathology.swmed.edu
Reply-To:
Date:Fri, 8 Oct 1999 10:14:37 -0600
Content-Type:text/plain; charset="us-ascii"

Thanks to everyone replying to my 10/7 question.  I was not clear in
stating my question.
        I am developing a virus based gene therapy/gene delivery model for
the mammary epithelium.  I have been successful in demonstrating the innate
fluorescent signal from the green fluorescent protein reporter gene product
(the GFP).  I want to be able to see the innate fluorescent signal from the
green fluorescent reporter gene product in transduced cells and to
simultaneously be able to see milk proteins in these same cells using
immunofluorescence techniques long established in my lab.  Specifically,
can the innate fluorescence from the GFP withstand the detergent and NaBH4
that I have been using in the immunofluorescent detection of milk proteins?


Neal






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