Cerb (her2) staining in a bone marrow biopsy

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From:Tom Wells <tomers@home.com>
To:'histonet' <histonet@pathology.swmed.edu>
Date:Wed, 13 Oct 1999 18:14:06 -0700
Content-Type:text/plain; charset="iso-8859-1"

I have a question regarding cerb (her2) staining. We are using the Dako
polyclonal antibody to cerb and we are very pleased with the results.
However, I was wondering if anybody has any experience with the effects of
(1) decalcification with HCl and (2) B5 fixation on cerb staining. We want
to stain mets in a bone marrow, unfortunately, we don't have access to the
primary breast tissue. So we have no way of checking our results.  Any
comments would be appreciated!  Thanks. Tom

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