saccomano's fixative

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From:Pat Stockton <> (by way of histonet)
To:histonet <>
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Would love to hear from anyone who has used/heard of Saccomano's (sp?)
Big thanks!

>Mime-Version: 1.0
>Date: Mon, 19 Oct 1998 09:37:21 -0400
>From: Julie Foley <>
>Subject: saccomano's fixative
>Can you do a posting on the histo site and see if you get any info on this
>one for Rob?
>>X-Sender: (Unverified)
>>Date: Sun, 18 Oct 1998 23:54:25 -0400
>>To: Julie Foley <>
>>From: Robbert Slebos <>
>>Subject: saccomano's fixative
>>Mime-Version: 1.0
>>Pardon my ignorance here, but I've looked and not found any decent recipe
>>for what's called Saccomano's fixative. My main interest is in the presence
>>of any cross-linkers that could mutilate our precious DNA <g>. It's for
>>bronchial washings, so not too many enzymes or other gooey stuff expected.
>>I was thinking of simply putting it in 10-20% ethanol.
>>Robbert J.C. Slebos, Ph.D.
>>Visiting Scientist
>>National Institute of Environmental Health Sciences
>>Laboratory of Molecular Carcinogenesis
>>Maildrop C2-01
>>P.O.Box 12233
>>Research Triangle Park, NC 27709
>>Telephone: 919-541-2694
>>Facsimile: 919-541-2511

Patricia Stockton, HT

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