re: lead (in bone)
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From: | Yvan Lindekens <yvan.lindekens@skynet.be> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Mr Capela e Silva:
A protocol for the detection of leadphosphate in bone after intravenous
injection of leadacetate can be found in:
Schneider B J (1968): "Lead acetate as a vital marker for the analysis of
bone growth". Amer J phys Anthropol 29 : 197 - 200.
Perhaps you can use it... The method involves fixation in NBF or
ethylalcohol 95%, decalcification in 1% hydrochloric acid with a constant
flow of H2S, cryosectioning, staining in goldchloride and mounting in
glycerin jelly. Can't give you a detailled protocol: don't have it...
Detection of lead in "soft tissues": lead salts are precipitated as
leadchromate after fixation in Regaud's fixative. These precipitates are
said to be "easy recognizable". The method is said to be sensitive and
specific...
Yvan Lindekens.
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