Up until recently, I have used my primary Ab diluent as my negative
control.  All of the buzz a few weeks ago convinced me that it was a good
idea to use sera from the animal the primary was made in.  Using the
control antibody at the same dilution as my primary I am now seeing
staining.  However, I see only what I would expect to see labelled with my
primary Ab.  Does this mean that I have to futz around with my conditions
until I see no labelling using my negative control antibody even though I
see a total different staining pattern than that of my primary?

Thanks again!!!!

Nora

Nora Fox
Division of Orthopaedic Surgery
Stanford University
Edwards R144, MC 5341
300 Pasteur Drive
Stanford, CA 94305
phone: (650)725-2746
fax:  (650)723-6396