negative controls

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From:Nora Fox <nefox@leland.Stanford.EDU> (by way of histonet)
To:histonet <>
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Up until recently, I have used my primary Ab diluent as my negative
control.  All of the buzz a few weeks ago convinced me that it was a good
idea to use sera from the animal the primary was made in.  Using the
control antibody at the same dilution as my primary I am now seeing
staining.  However, I see only what I would expect to see labelled with my
primary Ab.  Does this mean that I have to futz around with my conditions
until I see no labelling using my negative control antibody even though I
see a total different staining pattern than that of my primary?

Thanks again!!!!


Nora Fox
Division of Orthopaedic Surgery
Stanford University
Edwards R144, MC 5341
300 Pasteur Drive
Stanford, CA 94305
phone: (650)725-2746
fax:  (650)723-6396

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