amyloidosis

<< Previous Message | Next Message >>
From:Heike Grabsch <h.grabsch@uni-koeln.de> (by way of histonet)
To:histonet <histonet@magicnet.net>
Reply-To:
Content-Type:text/plain; charset="iso-8859-1"

I need some help may be practical or intellectual...

I have performed an autopsy on a man who died from cardiac arrhythmias
(otherwise healthy), but thatís not the point.
I find congo red positive extracellular material (apple green with
polarized light, like a text book case) in heart, lungs, spleen, kidney
etc (all tissues I looked at) in the vascular walls as well as in the
interstitium.
I tried to clasify this form of amyloidosis by immunocytochemistry using
kappa, lambda, beta2-microglobulin and amyloid A - antibodies: everything
was negative with positive controls run on the same slide.
Can this be real or is it a technical problem?
and then: what sort of amyloidois is this? There is no multiple myeloma
(I looked at the bone marrow), there are no diagnostic findings in serum
or urine. The only chronic infection is a mild cholecystitis.

any suggestions?

thanks for your help,

Dr. Heike Grabsch
Dep. of Pathology
University of Duesseldorf
Moorenstrasse 5
40225 Duesseldorf
Germany




<< Previous Message | Next Message >>