I am forwarding this message for Kim as she is having trouble posting to
histonet. Please respond directly to her or post on the net. Thanks

Victor Tobias
Manager Tissue Processing Lab
Washington Animal Disease Diagnostic Lab
Washington State University
Pullman Washington 99164-7034
(509) 335-5590
(509) 335-7424 fax
tobiasv@vetmed.wsu.edu

---------- Forwarded message ----------
Date: Fri, 30 Oct 1998 09:57:55 -0900
From: Kim DeRuyter <fnksd1@uaf.edu>
To: tobiasv@vetmed.wsu.edu
Subject: Thin Brain sections

Victor,
I've been trying to post a question on histonet that isn't getting thru.  I
will keep trying but in the mean time I thought you might have some good
ideas.  A researcher hear wants parraffin sections of mouse brain cortex.
The cortex is sliced into 350 micron sections exposed to different
treatments, then fixed and processed as usual.  The problem is that these
thin sections warp during processing making it impossible to get a complete
section of the tissue.  Is it possible to keep the trimmed sections flat
during processing?  I have never used agar.  Would embedding in agar first
work?  Or sandwiching in sponges?  Any ideas?
Thanks much.
Kim DeRuyter
Veterinary Services
UAF