Thin Brain sections (fwd)

<< Previous Message | Next Message >>
From:Ian Montgomery <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

 A researcher hear wants parraffin sections of mouse brain cortex.
>The cortex is sliced into 350 micron sections exposed to different
>treatments, then fixed and processed as usual.  The problem is that these
>thin sections warp during processing making it impossible to get a complete
>section of the tissue.  Is it possible to keep the trimmed sections flat
>during processing?  I have never used agar.  Would embedding in agar first
>work?  Or sandwiching in sponges?  Any ideas?
>Thanks much.
>Kim DeRuyter
>Veterinary Services

	I sandwich the slices between tissue processing foam pads, place in
a cassette and process in the normal way.

<< Previous Message | Next Message >>