Thin Brain sections (fwd)
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| From: | Ian Montgomery <I.Montgomery@bio.gla.ac.uk> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
A researcher hear wants parraffin sections of mouse brain cortex.
>The cortex is sliced into 350 micron sections exposed to different
>treatments, then fixed and processed as usual. The problem is that these
>thin sections warp during processing making it impossible to get a complete
>section of the tissue. Is it possible to keep the trimmed sections flat
>during processing? I have never used agar. Would embedding in agar first
>work? Or sandwiching in sponges? Any ideas?
>Thanks much.
>Kim DeRuyter
>Veterinary Services
>UAF
>
Kim,
I sandwich the slices between tissue processing foam pads, place in
a cassette and process in the normal way.
Ian.
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