Thin Brain sections (fwd)

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From:Ian Montgomery <I.Montgomery@bio.gla.ac.uk> (by way of histonet)
To:histonet <histonet@magicnet.net>
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 A researcher hear wants parraffin sections of mouse brain cortex.
>The cortex is sliced into 350 micron sections exposed to different
>treatments, then fixed and processed as usual.  The problem is that these
>thin sections warp during processing making it impossible to get a complete
>section of the tissue.  Is it possible to keep the trimmed sections flat
>during processing?  I have never used agar.  Would embedding in agar first
>work?  Or sandwiching in sponges?  Any ideas?
>Thanks much.
>Kim DeRuyter
>Veterinary Services
>UAF
>

Kim,
	I sandwich the slices between tissue processing foam pads, place in
a cassette and process in the normal way.
Ian.




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