Dear Pat,
we routinely use Saccomano's fixative esp with lab sites off campus; but we
do not use Saccomano's homogenising technique.
The fixative ready to use can be either a 5% Carbowax in 50% ethanol (or
100IMS), or a 2% Carbowax in 50% ehtanol.
Both are prepared from a 50% stock Carbowax solution in 50% Ethanol,
comprising Polyethylene Glycol MW.1500 approx (avoid using heavier mw's as
these do not penetrate as well)- and is most easily achieved by microwaving
the PEGS until it liquifies and then pouring it into and equal volume of 50%
Ethanol , all the while mixing rapidly to ensure solution.
Use the 5% working solution for liquid specs. cytology esp. urines and other
fliuds, using approx an equal volume fixative to sample. Use the 2% for
Sputa or even for smears made on site that you intend to later let dry and
transport back to your main lab.
We do emphasise however we only use these procs. to enable sites to send
material back to our main lab. (might take one or more days to arrive from
remote areas). We still prefer to use 95% for all material received fresh on
site with the execption of air dried smears and FNA's.
Hope this helps, Regards- Mike Rentsch (Downunder)
-----Original Message-----
From: Pat Stockton <stockton@niehs.nih.gov>
To: Histonet@pathology.swmed.edu <Histonet@pathology.swmed.edu>
Date: Thursday, 22 October 1998 12:17
Subject: saccomano's fixative


>Would love to hear from anyone who has used/heard of Saccomano's (sp?)
>fixative.
>Big thanks!
>pat
>
>>X-Sender: foley1@pop.niehs.nih.gov
>>Mime-Version: 1.0
>>Date: Mon, 19 Oct 1998 09:37:21 -0400
>>To: STOCKTON@niehs.nih.gov
>>From: Julie Foley <foley1@niehs.nih.gov>
>>Subject: saccomano's fixative
>>
>>Pat,
>>Can you do a posting on the histo site and see if you get any info on this
>>one for Rob?
>>Thanks
>>Julie
>>
>>
>>
>>>X-Sender: slebos@drucker.niehs.nih.gov (Unverified)
>>>Date: Sun, 18 Oct 1998 23:54:25 -0400
>>>To: Julie Foley <foley1@niehs.nih.gov>
>>>From: Robbert Slebos <slebos@niehs.nih.gov>
>>>Subject: saccomano's fixative
>>>Cc: slebos@niehs.nih.gov
>>>Mime-Version: 1.0
>>>
>>>Julie,
>>>
>>>Pardon my ignorance here, but I've looked and not found any decent recipe
>>>for what's called Saccomano's fixative. My main interest is in the
presence
>>>of any cross-linkers that could mutilate our precious DNA <g>. It's for
>>>bronchial washings, so not too many enzymes or other gooey stuff
expected.
>>>I was thinking of simply putting it in 10-20% ethanol.
>>>
>>>Thanks,
>>>Rob.
>>>
>>>----------------------------------------------------------------------
>>>Robbert J.C. Slebos, Ph.D.
>>>Visiting Scientist
>>>National Institute of Environmental Health Sciences
>>>Laboratory of Molecular Carcinogenesis
>>>Maildrop C2-01
>>>P.O.Box 12233
>>>Research Triangle Park, NC 27709
>>>
>>>Telephone: 919-541-2694
>>>Facsimile: 919-541-2511
>>>E-mail: slebos@niehs.nih.gov
>>>----------------------------------------------------------------------
>>>
>>
>
>Patricia Stockton, HT
>Stockton@niehs.nih.gov
>
>
>