Re: old slides
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From: | Katri Tuomala <katri@istar.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Hal,
On occasion I have had to rejuvenite old H&E slides. Soak them in xylene
until coverslips come off without too much prying (this may take days).
Take slides back to 70 % ethanol and give them a few minutes in 1% acid
alcohol to remove most of the old staining. Wash well in tap water and
restain with your routine H&E stain. This should solve your problem.
Katri Tuomala
St,Joseph's Hospital
Hamilton, ON, Canada
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