Re: old slides

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From:Katri Tuomala <> (by way of histonet)
To:histonet <>
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Hi Hal,
On occasion I have had to rejuvenite old H&E slides. Soak them in xylene
until coverslips come off without too much prying (this may take days).
Take slides back to 70 % ethanol and give them a few minutes in 1% acid
alcohol to remove most of the old staining. Wash well in tap water and
restain with your routine H&E stain. This should solve your problem.

Katri Tuomala
St,Joseph's Hospital
Hamilton, ON, Canada

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