Re: Unsubscribe

<< Previous Message | Next Message >>
From:Kruno Lorkovic <swansong@videotron.ca> (by way of histonet)
To:histonet <histonet@magicnet.net>
Reply-To:
Content-Type:text/plain; charset="us-ascii"


-----Original Message-----
From: HistoNet Server <HistoNet@Pathology.swmed.edu>
To: HistoNet Server <HistoNet@pathology.swmed.edu>
Date: Wednesday, October 28, 1998 2:09 AM
Subject: Daily Digest


>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 00:29:32 -0500
>From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
>Subject: Re: Dent's fixative
>
>On Mon, 26 Oct 1998, Karen D. Larison wrote:
>
>> I've been asked about Dent's fixative.  Does anyone know what this is?
>
>Stuff to prevent false teeth from falling out?
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 05:19:31 -0500
>From: Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
>Subject: Gaye's Solution/Fixative??
>
>I've been asked about Gaye's fixative.  Does anyone know what this is?
>
>- --
>Bruce Abaloz
>HISTOLOGIST
>Department of ZOOLOGY        *  ph:    +61 3 93446282
>The University Of Melbourne    *  fax:   +61 3 93447909
>Parkville Victoria.3052        *  email: b.abaloz@zoology.unimelb.edu.au
>
>AUSTRALIA.
>                    IF YOU WANT TO BE HAPPY......BE!!
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 05:21:24 -0500
>From: Pierre Mainil <mainil@patho.unibe.ch>
>Subject: Cartilage Decalcification and embedding
>
>Dear all,
>
>I want to thank everyone who responded to my question.  I am sure some
>tricks will work. I will start to try with the positively charged
>slides.
>Concerning the soakink I'm little bit affraid about possible interaction
>with immunohistochemistry.
>Does some once has a detail protocol for SAF O/Fast Green ?
>Does some known where anti col X (human) are available from?
>Once again, thank you all for your generous responses!
>
>
>Pierre Mainil-Varlet, MD, PhD
>Dept of pathology
>University of Bern
>Switzerland
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 05:22:05 -0500
>From: "Mick Rentsch" <ausbio@nex.com.au>
>Subject: Re: Clinical Notes
>
>Try "KRAFT SYNDROME":- Can't remember a flippin' thing!
>- -----Original Message-----
>From: Hawkins, Hal <hhawkins@SBI.UTMB.EDU>
>To: histonet <histonet@pathology.swmed.edu>; Mick Rentsch
><ausbio@nex.com.au>
>Date: Tuesday, 27 October 1998 2:06
>Subject: RE: Clinical Notes
>
>
>>
>>My favorite is the patient's answer to almost all questions on
>>the student's history and physical:
>>
>>NAIKO = Not As I Knows Of
>>
>> ----------
>>From:  Mick Rentsch
>>Sent:  Thursday, October 22, 1998 10:30 PM
>>To:  histonet
>>Subject:  Clinical Notes
>>
>>Dear Histonetters,
>>(and to those about to become histonutters). From time to time we can all
>>have a bit of chuckle on the open channel like we all did with the saga's
>>for the "Missing Specimens" and "Labcoats".
>>Well, it's time for another round of hillarity.
>>How about those clinical notes we all get from time to time? eg:_-"GOK"
>>being God only knows
>>or HASOAD having a S of a day.
>>Come on, put in your 2 bobs worth (Sorry twenty cents worth)
>>Mike Rentsch
>>
>>
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 05:22:42 -0500
>From: "Mick Rentsch" <ausbio@nex.com.au>
>Subject: Re: Fast Drying Mounting Medium
>
>Dear Joseph,
>two mediums that come to mind are "Eukitt" and "Entellan Neu". When I
worked
>in Cyto we used Eukitt extensively along with Entellan Neu (New) because
>they were rapid setting and allowed oil immersion if necessary with in
20-30
>minutes. We noted fading on storage with the Eukitt. Later when I went into
>Histo we continued to use Entellan  (For the last 12 yrs or so), we have'nt
>noted any apprecciable fading, and found that at room temperature we could
>file all slides at day three or four without sticking and if we used a
>warming tray they were filed the next morning. We also used the slide
warmer
>to "harden off" the slides same day if we had to send them off for any
>reason (2-3hrs was sufficient @ 45-50C). But as I said earlier the
coverslip
>was hard enough  at 20-30 mins, to withstand oil immersion and wiping off
>the oil, and of course also spot marking suspect cells, clumps etc without
>the coverslip moving.
>The Entellan Neu can be purchased from EM Scientific in the states and
>BDH/Merck everywhere else. As for Eukitt, I've long since lost the
>address/company name.
>regards Mike Rentsch (Downunder)
>- -----Original Message-----
>From: Saby, Joseph <Joseph.Saby@wl.com>
>To: 'HistoNet@pathology.swmed.edu' <HistoNet@pathology.swmed.edu>
>Date: Tuesday, 27 October 1998 10:39
>Subject: Fast Drying Mounting Medium
>
>
>>Fellow Histonetters-
>>
>>We are looking for a fast drying mounting medium.  The problem is that
>>we turn our slides in to the pathologists in racks in which the slides
>>are held upright.  With permount, even after a week of drying there are
>>times the slides will get all stuck together. This mounting medium has
>>to be compatible with a Hacker(R) Robotic Coverslipper.
>>
>>Thanks in advance for suggestions!
>>
>>Joe Saby, BS HT
>>Parke-Davis, Ann Arbor, MI.
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 06:31:44 -0500
>From: "Saby, Joseph" <Joseph.Saby@wl.com>
>Subject: RE: Clinical Notes
>
>Mick-
>
>Thanks for the input on mounting media!  "Craft" syndrome is called CRS
>syndrome in the states-
>Can't Remember S#*t.
>
>Joe Saby, BA HT
>Parke-Davis, Ann Arbor, MI
>
>> -----Original Message-----
>> From: Mick Rentsch [SMTP:ausbio@nex.com.au]
>> Sent: Tuesday, October 27, 1998 2:11 AM
>> To: Hawkins, Hal
>> Cc: histonet@pathology.swmed.edu
>> Subject: Re: Clinical Notes
>>
>> Try "KRAFT SYNDROME":- Can't remember a flippin' thing!
>> -----Original Message-----
>> From: Hawkins, Hal <hhawkins@SBI.UTMB.EDU>
>> To: histonet <histonet@pathology.swmed.edu>; Mick Rentsch
>> <ausbio@nex.com.au>
>> Date: Tuesday, 27 October 1998 2:06
>> Subject: RE: Clinical Notes
>>
>>
>> >
>> >My favorite is the patient's answer to almost all questions on
>> >the student's history and physical:
>> >
>> >NAIKO = Not As I Knows Of
>> >
>> > ----------
>> >From:  Mick Rentsch
>> >Sent:  Thursday, October 22, 1998 10:30 PM
>> >To:  histonet
>> >Subject:  Clinical Notes
>> >
>> >Dear Histonetters,
>> >(and to those about to become histonutters). From time to time we can
>> all
>> >have a bit of chuckle on the open channel like we all did with the
>> saga's
>> >for the "Missing Specimens" and "Labcoats".
>> >Well, it's time for another round of hillarity.
>> >How about those clinical notes we all get from time to time?
>> eg:_-"GOK"
>> >being God only knows
>> >or HASOAD having a S of a day.
>> >Come on, put in your 2 bobs worth (Sorry twenty cents worth)
>> >Mike Rentsch
>> >
>> >
>> >
>> >
>>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 08:19:46 -0600
>From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
>Subject: RE: Dent's fixative
>
>Karen:
>
>Dent's cold fixative is used for some fluorescence labeling techniques:
>
>1 part (DMSO) dimethyl sulfoxide
>4 parts methanol
>
>Stored in the freezer at -20 degree centigrade for Blastogenetics, or in
the
>cryostat on frozen sections for Immunofluorescence.
>
>Eric Kellar
>Histology/Immunohistochemistry
>University of Pittsburgh Medical Center
>
> ----------
> From:  Karen D. Larison [SMTP:LARISONK@UONEURO.uoregon.edu]
> Sent:  Monday, October 26, 1998 6:22 PM
> To:  HistoNet@Pathology.swmed.edu
> Subject:  Dent's fixative
>
> Histonetters,
>
> I've been asked about Dent's fixative.  Does anyone know what this
>is?
>
> Thanks a bunch.  -Karen
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 09:57:19 -0600
>From: Valerie DeGroff <degroff.1@osu.edu>
>Subject: Re: foil labelers
>
>Since I have been investigating a lot of equipment for our path lab, I
>have become familiar with many catalogs.
>In the IMEB (800-543-8496) catalog is listed a TBS SHUR/MARK cassette
>labeler (cat# H-SM-CE).  It describes "a heated stylus scribes through
>hot foil tape to produce permanently printed cassettes impervious to
>processing reagents".  This may be what you are referring to.
>I would also like to thank all of those people who so kindly and
>patiently responded to my inquiries.
>Have a nice day!
>Valerie DeGroff
>Ohio State University
>
>Hagerty, Marjorie A. wrote:
>
>> Histonetters,
>> During the discussion regarding cassette labeling, Rebecca S. Smith
>> said,
>> "The ultimate however are the foil cassette labeling machines." What
>> is
>> this? We have a Surgipath cassette labeler and have not heard it
>> referred to
>> it as a foil labeler machine. Is there another cassette labeling
>> machine out
>> there? Thanks in advance.
>> Marg
>> EMC, Rancho Mirage, CA, USA
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 10:00:10 -0600
>From: "Tim Morken" <timcdc@hotmail.com>
>Subject: Re: negative controls
>
>Nora,
>
>Normal serums have a lot more protein and antibodies than the antibody
>you buy. Most commercial antibodies have been affinity purified, which
>means that almost all that is left is the antibody of interest and some
>proteins. If you are using monoclonal Fab fragments you should see no
>background at all. Ascitic fluids will have polyclonal antibodies and
>some proteins, but not as much as serum. So commercial preps are usually
>very clean.
>
>The control serum is intended to show non-specific staining (NSS) that
>could be caused by the serum in the primary. If the pattern is totally
>different it is not necessarily bad, especially if the pattern is
>different. If the NSS is too heavy, however, you may want to check your
>serum protein concentration (may be too high) and/or filter the serum to
>take out complexes of protein that may have formed (use a .22 um
>filter).
>
>Another thing to do is use non-specific antibodies in the same form
>(poly, mono, ascitic fluid) and from the same animal species as
>negatives. This may give you a truer picture of NSS.
>
>If you still have problems, review your blocking procedures.
>
>Tim Morken, B.S., EMT(MSA), HTL(ASCP)
>Infectious Disease Pathology
>Centers for Disease Control
>MS-G32
>1600 Clifton Rd.
>Atlanta, GA 30333
>USA
>
>email: tim9@cdc.gov
>       timcdc@hotmail.com
>
>FAX:  (404)639-3043
>
>
>- ----Original Message Follows----
>Date: Fri, 23 Oct 1998 14:52:13 -0700
>From: Nora Fox <nefox@leland.Stanford.EDU>
>Subject: negative controls
>To: histonet@pathology.swmed.edu
>
>Up until recently, I have used my primary Ab diluent as my negative
>control.  All of the buzz a few weeks ago convinced me that it was a
>good
>idea to use sera from the animal the primary was made in.  Using the
>control antibody at the same dilution as my primary I am now seeing
>staining.  However, I see only what I would expect to see labelled with
>my
>primary Ab.  Does this mean that I have to futz around with my
>conditions
>until I see no labelling using my negative control antibody even though
>I
>see a total different staining pattern than that of my primary?
>
>Thanks again!!!!
>
>Nora
>
>Nora Fox
>Division of Orthopaedic Surgery
>Stanford University
>Edwards R144, MC 5341
>300 Pasteur Drive
>Stanford, CA 94305
>phone: (650)725-2746
>fax:  (650)723-6396
>
>
>
>
>
>
>______________________________________________________
>Get Your Private, Free Email at http://www.hotmail.com
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 10:01:42 -0600
>From: "Margaret Gondo" <gondom@genemedicine.com>
>Subject: Freezing Muscle
>
>I just wanted to send out a big THANK YOU to everyone who answered my post.
>
>You all are a great bunch of people!
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 11:19:55 -0600
>From: "Steven E. Slap" <ebs@ebsciences.com>
>Subject: Re: Ink pads
>
>Hi HistoNetters!
>
>Michelle Skelton asked:
>>Does anybody know who sells the ink pads that can be used with the
"marking
>>objective?" on a microscope.  I know they use them quite a bit in cytology
>>to put a little circle of ink around suspicious areas on the slides.
Regular
>>ink pads don't work because they smear on the coverslip.  Thanks in
advance.
>
>First, remember, I'm a *vendor*...(boo!hiss!)
>
>We manufacture a product called the "Slide Marker" which fits the
>description above.  We also sell the individual components of the slide
>marker kit, including the ink pads.
>
>End commercial.
>
>Best regards,
>Steven E. Slap, Vice-President
>
>
>********************************
>Energy Beam Sciences, Inc.
>The Laboratory Microwave Company
>http://www.ebsciences.com
>********************************
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 11:20:25 -0600
>From: Lynn Gardner <gardnerl@horus.ophth.uiowa.edu>
>Subject: Sliding microtome
>
> To all concerned,
>
>Does anyone have or know of anyone who has a sliding microtome they are
>wanting to get rid of or anyone who would cut tissue sections of breast
>tissue on a sliding microtome? Need the answers as soon as possible.
>
>Thanks all for your help!
>Lynn Gardner
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 11:20:55 -0600
>From: Cheryl Crowder <crowder@vt8200.vetmed.lsu.edu>
>Subject: cassettes
>
>Histonetters - I just received my order from Fisher for the
>compartmentalized biopsy cassettes.  The cassettes are made by Evergreen
and
>sold through Fisher.  The numbers are as follows since they are not yet in
>all their computers:
>        258-4254-H90    2 compartment biopsy cassette - gray    500/case
>        258-4266-H90    4 compartment biopsy cassette - gray    500/case
>        258-4281-H90    9 compartment biopsy cassette - gray    500/case
>All were $70.00/case..
>
>Hope this helps some of you talk to Fisher.  Cheryl
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 11:21:23 -0600
>From: "Tarpley, John" <jtarpley@amgen.com>
>Subject: RE: Leitz 1512 knifeholder
>
>Yes, it's an excellent holder. It's very reliable since it does not contain
>springs or other moving parts. It simply relies on the clamping pressure of
>the microtome clamping screws. It is specific to the type of microtome so
it
>may not be available for all types of microtomes. I've used one for several
>years. In fact, I have one of the original prototypes that's still in
>service. Klaus originally worked for the Leitz factory before coming to the
>US and locating his microtome repair business in Atlanta.
>
>John Tarpley 15-2-B
>Specialist Image Analysis & Immunohistochemistry
>Amgen Inc.
>One Amgen Center Drive
>Thousand Oaks, CA  91320
>
>Views expressed are mine alone and do not represent the views of my
employer
>
>
>> ----------
>> From: Gayle Callis[SMTP:uvsgc@msu.oscs.montana.edu]
>> Sent: Monday, October 26, 1998 1:14 PM
>> To: histonet@Pathology.swmed.edu
>> Subject: Leitz 1512 knifeholder
>>
>> Joyce Weems put out info on the Klaus Dern knifeholder, it is creme de
>> la creme, can be autoclaved (for those who use it in a cryostat), and
>> extremely sturdy. He makes them for other microtomes, not just the
>> Leitz/Leicas.  It is called the K-D knifeholder.  If John Tarpley
>> is listening in, he will attest to the quality of this holder.
>> Nice to know he is still in business.
>>
>> Gayle Callis
>>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 12:08:56 -0600
>From: "Alexander Nader" <anader@teleweb.at>
>Subject: Thank You All
>
>- -----BEGIN PGP SIGNED MESSAGE-----
>
>After a year of struggle with the owners of the hospital I'm working, it
was
>now possible to
>get an internet connection too. One of the major things that impressed them
>was the
>answers and discussions of this list. I think it was the first time that
they
>recognised that
>there are other things than sex on the internet.
>
>
>
>
>
> Alex Nader
> anader@teleweb.at
>PGP Public Key Fingerprint = FC 6C AD 66 8C 93 17 6F  3F 38 2E 7F 2A 96 AA
E4
>
>- -----BEGIN PGP SIGNATURE-----
>Version: 2.6.3i
>Charset: noconv
>
>iQCVAwUBNjYTbm11cRuLAsYVAQHyEQQAlK2GZULtOE67T9nsTvrwQTAD74VIQIcd
>N/TMUHPVfyMN6J0yZC80y6vmHiMtxJ1c8uTQxf+J35wWYtrJKZjt+JhkSS+CngiO
>DO4ZgB07XcEbLC5sNyVpdFnSt/mcqp/qRxbGnO81PA/15nBk9QW+NgPP7OOrEVnA
>JRBd3ZdP8u0=
>=9kFw
>- -----END PGP SIGNATURE-----
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 13:45:55 -0600
>From: "Baileyhealy, Irene {USA~Palo Alto}" <IRENE.BAILEYHEALY@roche.com>
>Subject: RE: alk phos staining/kits
>
>Hi Nora/Gayle
>One of my co-workers successfully used Vector's alk phos substrate kit
>(Vector red) to stain cultured cells which express alk phos.
>Irene
>CA
>USA
>
> -----Original Message-----
> From: Gayle Callis [SMTP:uvsgc@msu.oscs.montana.edu]
> Sent: Monday, October 26, 1998 6:30 PM
> To: histonet@pathology.swmed.edu
> Subject: alk phos staining/kits
>
> Nora,
> Donna's suggestion for alk phos was excellent, and this is a what if
> question, or can you:  Why not use an alkaline phosphatase kit,
> say Vectors, to stain the cells?  so easy and inexpensive, and also
> is anyone doing this or tried it?
> This is the same kit used for alk phos immunostaining. All
>ingredients are
> present, ready to go.
>
> Gayle Callis
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 13:46:17 -0600
>From: Hedley David Glencross <hedley@hheath.demon.co.uk>
>Subject: ink pads
>
>Hi
>
>I had trouble finding these as they were withdrawn from sale in the UK.
>After much searching, via some help from Merck UK, I obtained some from
>Leica Sweden, their last seven in fact. They did however have some
>complete sets of object markers, including pads. Perhaps you can try
>them. Your agents for Leica should be able to get the address.
>
>If that fails, I know that Nikon sell an object marking lens, apparently
>including one compatible with the Eclipse series too.
>
>Hope this helps.
>
>Regards.
>- --
>Hedley David Glencross
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 13:46:40 -0600
>From: "Bartlett, Jeanine" <jqb7@cdc.gov>
>Subject: Vashaw
>
>Can anyone help me with an email address for Wayne Vashaw?
>
>Thanks in advance!
>Jeanine Bartlett, HT (ASCP)
>CDC, NCID, DVRD, IDPA
>(404) 639-3590
>jqb7@cdc.gov
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 13:47:05 -0600
>From: Nora Fox <nefox@leland.Stanford.EDU>
>Subject: Re: double labeling
>
>I would like to see this too--as may lots of others.
>
>Thank you to all of you who have responded to my many questions.
>
>Nora
>
>>
>>>Good Morning,
>>>        Would someone who has some experience with double labeling[IHC]
>>>please e-mail me directly? I have a few questions from one of my
>>>colleagues...we're interested in one Ab w/DAB and one with alk.phos.
Thanks
>>> Best Regards,
>>> Mary Vaughan HT(ASCP)
>>> Roswell Park Cancer Institute
>>> Pharmacology + Therapeutics
>>> Elm + Carlton Sts.  CDC-121
>>> Buffalo, NY 14263
>>
>>
>>
>Nora Fox
>Division of Orthopaedic Surgery
>Stanford University
>Edwards R144, MC 5341
>300 Pasteur Drive
>Stanford, CA 94305
>phone: (650)725-2746
>fax:  (650)723-6396
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 14:26:29 -0600
>From: Nora Fox <nefox@leland.Stanford.EDU>
>Subject: Re: alk phos staining/kits
>
>Thanks, Gayle.  Have you tried using the kit for this purpose?  Has anyone
>else out there?
>
>>Nora,
>>Donna's suggestion for alk phos was excellent, and this is a what if
>>question, or can you:  Why not use an alkaline phosphatase kit,
>>say Vectors, to stain the cells?  so easy and inexpensive, and also
>>is anyone doing this or tried it?
>>This is the same kit used for alk phos immunostaining. All ingredients are
>>present, ready to go.
>>
>>Gayle Callis
>
>Nora Fox
>Division of Orthopaedic Surgery
>Stanford University
>Edwards R144, MC 5341
>300 Pasteur Drive
>Stanford, CA 94305
>phone: (650)725-2746
>fax:  (650)723-6396
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 14:26:56 -0600
>From: Phyllis Davie <pdavie@phenopath.com>
>Subject: Re: tumor differentials
>
>Hi,
>Sorry about the delay in responding.  There is a wonderful web site for
>PhenoPath Laboratories (yes, I work there!) at www.phenopath.com.  Go
>under the clinical services, and select the "differential" of your
>choice.  There are some beautiful algorithms developed by Dr. Allen Gown
>(my boss).  They are not flow charts per se, but it sounds like it would
>fit your bill to a T.  Besides, we have some pretty pictures there (in
>all modesty, of course).
>
>
>
>>hi all,
>>
>>i'm just sure there's someone out there who can help me.   i'm looking for
a
>>set of "flow charts" for differential diagnosing of various tumors using
>>ihc.  is there one reference or web site perhaps where i can find such a
>>thing.....i know there is.....just looking for a short-cut to getting my
>>hands on one.
>>thanks,
>>carrie kyle-byrne
>>eire@teleport.com
>>
>>
>
>
>Phyllis Davie
>Clinical Laboratory Supervisor
>PhenoPath Laboratories
>Seattle, WA
>pdavie@phenopath.com
>(206)374-9009  phone
>(206)374-9009  fax
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 15:42:37 -0600
>From: "Weems, Joyce" <JWEEMS@sjha.org>
>Subject: RE: Thank You All
>
>Well, don't tell them about Ohio's 'Erotic Series"! :>)
>
>>----------
>>From: Alexander Nader[SMTP:anader@teleweb.at]
>>Sent: Tuesday, October 27, 1998 12:39 PM
>>To: Histology Net List Server
>>Subject: Thank You All
>>
>>-----BEGIN PGP SIGNED MESSAGE-----
>>
>>After a year of struggle with the owners of the hospital I'm working, it
was
>>now possible to
>>get an internet connection too. One of the major things that impressed
them
>>was the
>>answers and discussions of this list. I think it was the first time that
they
>>recognised that
>>there are other things than sex on the internet.
>>
>>
>>
>>
>>
>> Alex Nader
>> anader@teleweb.at
>>PGP Public Key Fingerprint = FC 6C AD 66 8C 93 17 6F  3F 38 2E 7F 2A 96 AA
E4
>>
>>-----BEGIN PGP SIGNATURE-----
>>Version: 2.6.3i
>>Charset: noconv
>>
>>iQCVAwUBNjYTbm11cRuLAsYVAQHyEQQAlK2GZULtOE67T9nsTvrwQTAD74VIQIcd
>>N/TMUHPVfyMN6J0yZC80y6vmHiMtxJ1c8uTQxf+J35wWYtrJKZjt+JhkSS+CngiO
>>DO4ZgB07XcEbLC5sNyVpdFnSt/mcqp/qRxbGnO81PA/15nBk9QW+NgPP7OOrEVnA
>>JRBd3ZdP8u0=
>>=9kFw
>>-----END PGP SIGNATURE-----
>>
>>
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 15:43:13 -0600
>From: "Sarah A. Jones" <hawkmoon15@earthlink.net>
>Subject: Steamers for IHC HIER
>
>Hello Histonet~
>
>I work for a facility that is about to demo a DAKO IHC Stainer.  The rep
>called us yesterday and told us that the CAP had just issued a new
>regulation stating that steamers could no longer be used for heat incuded
>epitope retreival (HIER).  He is in the process of getting the information
>for us, as well as the substitute method; but I have yet to see any
>documentation.  Has anyone else heard about this?  He apparently just
>learned about it himself last week-end, so it is something very new.  Will
>be anxious to get some feed-back.
>
>Thanks!
>Sarah A. Jones
>APMG
>Los Gatos, CA
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 16:27:37 -0600
>From: Karen K. Dulany <kkdulany@MAIL.UNMC.EDU>
>Subject: Re: double labeling -Reply
>
>Dear Nora, We do double staining a lot, and have even gone on to do
>triple and quadruple staining.  When you get 4 different antibodies
>and 4 different colors on one slide, it is very beautiful.  Time
>consuming but very pretty when all are positive for the antibodies
>you hope to see.  If I can be of further help, I will try to help
>you.
>Karen Dulany HTL (ASCP)
>Univ. of NE Medical Center
>Eppley Instiute for Cancer Research
>Omaha, NE
>402-559-5123
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 16:28:03 -0600
>From: John Difford <adford@compuserve.com>
>Subject: Re: Safranin O / Fast Green
>
>
>Re: Safranin O / Fast Green Method
>
>Attn.Dr Pierre Mainil-Varlet
>
>Dear Pierre,
>
>The Safranin O method for Cartilage goes like this;
>
>1. Dewax section and take to water
>2. Stain nuclei with a suitable iron haematoxylin
>3. Blue in running tapwater
>4. Rinse in distilled water
>5. Stain with 1% light green diluted 1 in 5 with distilled water, for 3
>minutes
>6. Rinse in 1% acetic acid
>7. Stain with 0.1% Saffranin O, for 4 - 6 minutes
>8. Rinse in 1% acetic acid and check under microscope. Any overstaining
>with Safranin can be modified by re-applying the light green     solution
>briefly, and vice versa.
>9. Dehydrate with alcohol,clear and mount
>
>Modified from the method in Lillie 'Histopathological Technic and Practical
>Histochemistry'
>
>Regards
>
>John Difford FIBMS
>Histopathology Dept
>Royal Free Hospital
>London, England.
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 16:28:47 -0600
>From: "Sarah A. Jones" <hawkmoon15@earthlink.net>
>Subject: New CAP reg re: using Steamers for IHC??
>
>Hello Histonet ~
>
>I work for a facility that is about to demo a DAKO IHC Stainer.  The rep
>called us yesterday and told us that the CAP had just issued a new
>regulation stating that steamers could no longer be used for heat induced
>epitope retreival (HIER).  He is in the process of getting the information
>for us, as well as the substitute method; but I have yet to see any
>documentation.  Has anyone else heard about this?  He apparently just
>learned about it himself last week-end, so it is something very new.  Will
>be anxious to get some feed-back.
>
>Thanks!
>Sarah A. Jones
>APMG
>Los Gatos, CA
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 16:29:09 -0600
>From: Jill Songer <jtsonger@vt.edu>
>Subject: control tissue
>
>Hello fellow histonetters. Would any veterinary lab be interested in
sending
>me a block of Leptospirosis positive tissue? I could trade a block of
>positive copper or Zeihl Neelson (Johnes) or Amyloid. Thanks.
>
>
>
>**************************************************************************
>Jill Songer HT (ASCP)
>Veterinary Teaching Hospital
>Virginia Tech
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 17:10:06 -0600
>From: Tom Kuwahara <tom@adpath.com>
>Subject: Re: double labeling
>
>Dear Nora:  Mary had some specific questions on her samples but I can
>give you the same reference I gave her.  You need two different
>immunhistochemical systems and two different chromogens.  You can do
>sequential staining or simultaneous double staining depending on your
>choice of systems, abs, etc..  Color contrast is important.  My friend,
>Dr. Chris M Van der Loos in Amsterdam has done a lot of work with double
>and triple staining.  He has just finished writing up a manual for DAKO,
>Immunoenzymatic Double Staining Methods, which I found to be very
>helpful for those wanting to double stain things.  I would highly
>recommend this as a good starting place.  I assume the manual's free
>from your DAKO rep, right Christopher?  The usual disclaimers: I don't
>work for DAKO and you don't have to use DAKO products to make it work.
>Also double staining isn't necessarily more helpful for any "diagnostic"
>uses than staining two separate slides but may help in research work and
>looks great!
>Regards, Tom Kuwahara
>
>Nora Fox wrote:
>>
>> I would like to see this too--as may lots of others.
>>
>> Thank you to all of you who have responded to my many questions.
>>
>> Nora
>>
>> >
>> >>Good Morning,
>> >>        Would someone who has some experience with double labeling[IHC]
>> >>please e-mail me directly? I have a few questions from one of my
>> >>colleagues...we're interested in one Ab w/DAB and one with alk.phos.
>Thanks
>> >>                              Best Regards,
>> >>                              Mary Vaughan HT(ASCP)
>> >>                              Roswell Park Cancer Institute
>> >>                              Pharmacology + Therapeutics
>> >>                              Elm + Carlton Sts.  CDC-121
>> >>                              Buffalo, NY 14263
>> >
>> >
>> >
>> Nora Fox
>> Division of Orthopaedic Surgery
>> Stanford University
>> Edwards R144, MC 5341
>> 300 Pasteur Drive
>> Stanford, CA 94305
>> phone: (650)725-2746
>> fax:  (650)723-6396
>
>- --
>*******************************
>Thomas J. Kuwahara
>Senior Immunohistochemist
>Advanced Pathology Systems
>3801 Sacramento St. suite 621
>San Francisco, CA 94118
>415 750 6800 x23067 tel
>415 750 2332 fax
>tom@adpath.com
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 17:11:03 -0600
>From: Cindy Farman <cfarman@sierrabiomedical.com>
>Subject: RE: alk phos staining/kits
>
>Hi Nora,
>
>I used the Vector kit once for this purpose because I thought I had made up
>the substrate solution wrong on an IHC run. I happened to have some
>intestinal sections already on slides so I made up new solution with the
>kit and it was positive for endogenous alkaline phosphate in the
>intestines. I have not used it any more extensively than that, but the
>stain was plenty dark enough and unequivocal in that circumstance.
>
>Cindy Farman
>
>- -----Original Message-----
>From: Nora Fox [SMTP:nefox@leland.Stanford.EDU]
>Sent: Tuesday, October 27, 1998 11:50 AM
>To: histonet@Pathology.swmed.edu
>Subject: Re: alk phos staining/kits
>
>Thanks, Gayle.  Have you tried using the kit for this purpose?  Has anyone
>else out there?
>
>>Nora,
>>Donna's suggestion for alk phos was excellent, and this is a what if
>>question, or can you:  Why not use an alkaline phosphatase kit,
>>say Vectors, to stain the cells?  so easy and inexpensive, and also
>>is anyone doing this or tried it?
>>This is the same kit used for alk phos immunostaining. All ingredients are
>>present, ready to go.
>>
>>Gayle Callis
>
>Nora Fox
>Division of Orthopaedic Surgery
>Stanford University
>Edwards R144, MC 5341
>300 Pasteur Drive
>Stanford, CA 94305
>phone: (650)725-2746
>fax:  (650)723-6396
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 18:03:17 -0600
>From: Dave Cavanaugh <davec@stallion.vdl.iastate.edu>
>Subject: want to buy
>
>Wanted to Buy: Leica 2035 microtome,  e-mail reply or
>               call 515-294-1204. ask for Dave. dc
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 19:00:10 -0600
>From: "Connolly, Brett" <brett_connolly@merck.com>
>Subject: RE: double labeling
>
>Mary, Nora et al.
>KPL (Kirkegaard & Perry Laboratories) puts out a useful manual for
>peroxidase/alk. phos. double staining, complete with protocols.  Vector
>Laboratories also has double staining procedures available. I have  found
>this article to be useful; Practical suggestions for successful
immunoenzyme
>double-staining experiments. Van der Loos et al.,Histochemical J,25:1-13,
>1993.
>
>Brett
>
>Brett M. Connolly, Ph.D.
>Merck Research Laboratories
>WP26A-3000
>P.O. Box 4
>West Point, PA 19486
>ph.215-652-2501
>FAX. 215-652-2075
>email:brett_connolly@merck.com
>
>> ----------
>> From: Nora Fox
>> Sent: Tuesday, October 27, 1998 3:47 PM
>> To: histonet@pathology.swmed.edu
>> Subject: Re: double labeling
>>
>> I would like to see this too--as may lots of others.
>>
>> Thank you to all of you who have responded to my many questions.
>>
>> Nora
>>
>> >
>> >>Good Morning,
>> >>        Would someone who has some experience with double labeling[IHC]
>> >>please e-mail me directly? I have a few questions from one of my
>> >>colleagues...we're interested in one Ab w/DAB and one with alk.phos.
>> Thanks
>> >> Best Regards,
>> >> Mary Vaughan HT(ASCP)
>> >> Roswell Park Cancer Institute
>> >> Pharmacology + Therapeutics
>> >> Elm + Carlton Sts.  CDC-121
>> >> Buffalo, NY 14263
>> >
>> >
>> >
>> Nora Fox
>> Division of Orthopaedic Surgery
>> Stanford University
>> Edwards R144, MC 5341
>> 300 Pasteur Drive
>> Stanford, CA 94305
>> phone: (650)725-2746
>> fax:  (650)723-6396
>>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 19:00:35 -0600
>From: "vector@vectorlabs.com" <vector@vectorlabs.com>
>Subject: Re: double labeling
>
>For those interested in double (or triple) immunolabeling of antigens on
>the same tissue section, using a combination of peroxidase and/or
>alkaline phosphatase reagents, Vector Laboratories features a section
>about this on our website. There are about 20 color photomicrograph
>examples, some general considerations for this application, and a
>protocol. This information was featured in a Vector newsletter alittle
>while ago, but this is still available, and current, and will be mailed
>to those who would like to receive their own copy. Please contact Vector
>by phone, fax or e-mail to request a multiple antigen labeling Vectagram
>copy.
>
>Hopefully this will answer alot of questions about the application.
>
>Sincerely,
>
>
>Technical Services
>
>Vector Laboratories
>30 Ingold Road
>Burlingame, CA 94010
>
>Tel:  (650) 697-3600
>Fax:  (650) 697-0339
>E-mail:  vector@vectorlabs.com
>Website:  http://www.vectorlabs.com
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 19:00:58 -0600
>From: krenek@gtwn.net
>Subject: controls
>
>I have a question for anyone that is doing muscle enzyme stains.  My
>pathologist tells me I don't need to run a control the muscle will have
>an internal control.  The hospital has a CAP inspection coming up soon
>and I am afraid this will not be acceptable.  What do other labs do?  We
>do run controls for all IHC on muscles.  Can I just say in my procedure
>that the control is internal?
>
>Thanks for any help.
>
>Pam Krenek
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 19:01:38 -0600
>From: "Garcia, Vicki {USA~Palo Alto}" <VICKI.GARCIA@roche.com>
>Subject: RE: Muscle freezing: Temperature
>
>Tim/All-
>When you suspend the muscle from the wooden block, how do you get the
muscle
>to hang straight down?  I am freezing whole mouse soleus muscle and the
>muscle will not hang without curling!  Any suggestions?
>Also, for those of you who section fresh frozen muscle, what type of knife
>do you use?  I would like to hear opinions about C pofile knives -vs- B
>profile knifes and also disposable(what brand)?
>Thank you for your help!
>
>Vicki Garcia
>Roche Bioscience
>Palo Alto, Ca
>
>> -----Original Message-----
>> From: Tim Morken [SMTP:timcdc@hotmail.com]
>> Sent: Friday, October 23, 1998 3:06 PM
>> To: histonet@pathology.swmed.edu
>> Subject: Muscle freezing: Temperature
>>
>> The best way to get a consistent freezing  temperature of the isopentane
>> used in freezing muscle biopsies is to use a digital thermometer. We
>> used one from Fluka. The thermometer has a metal probe rod which can
>> also be used to stir the isopentane. We let the temperature go down to
>> neg 160 deg C and then plunged in the muscle sample for 25 seconds. It
>> is important, in my experience, to have the liquid nitrogen level be at
>> or above the level of the isopentane in the beaker in order to get the
>> best temperature drop.
>>
>> The muscle sample (human) was prepared by sectioning off a piece of a
>> biopsy and orienting it so we would get a cross section. A wood square
>> about 25 mm square and 5 mm thick was spread with a mix of 10 percent
>> gum tragacanth which was made into a pyramid shape. The muscle was put
>> on the tip of the pyramid and pushed into the gum a little bit to hold
>> it. We then held it upside down until the temp of the isopentane was
>> just right. This insured a good orientation. We used a pair of big
>> forceps to hold the block.
>>
>> After freezing we put it in a zip lock bag in the cryostat (if it was to
>> be sectioned that day) or the minus 70 deg C freezer (if it was to be
>> done later). Never had a single bad prep this way.
>>
>>
>> Tim Morken, B.S., EMT(MSA), HTL(ASCP)
>> Infectious Disease Pathology
>> Centers for Disease Control
>> MS-G32
>> 1600 Clifton Rd.
>> Atlanta, GA 30333
>> USA
>>
>> email: tim9@cdc.gov
>>        timcdc@hotmail.com
>>
>> FAX:  (404)639-3043
>>
>> ----Original Message Follows----
>> Date: Fri, 23 Oct 1998 10:09:35 -0700
>> From: "Garcia, Vicki {USA~Palo Alto}" <VICKI.GARCIA@roche.com>
>> Subject: RE: Freezing Muscle
>> To: 'Margaret Gondo' <gondom@genemedicine.com>,
>> histonet@pathology.swmed.edu
>>
>> Hi Margaret-
>> I just started freezing mouse soleus muscle and have tried everything!
>> The
>> procedure that works best for me is attaching the muscle to the chuck
>> using
>> gum tragacanth and then freezing it in isopentane cooled by liquid
>> nitrogen.
>> I suspend a stainless steel beaker above the container of liquid
>> nitrogen so
>> that the very bottom of the beaker is submerged in the liquid nitrogen.
>> It
>> is critical that your isopentane is cold enough.  The isopentane will
>> begin
>> to turn white at the bottom of the container and will begin to get
>> viscous.
>> At this point it is cold enough to freeze muscle.
>>
>> Vicki Garcia
>> Roche Bioscience
>> Tissue Repair
>> Palo Alto, Ca.
>> > -----Original Message-----
>> > From: Margaret Gondo [SMTP:gondom@genemedicine.com]
>> > Sent: Friday, October 23, 1998 9:39 AM
>> > To: histonet@pathology.swmed.edu
>> > Subject: Freezing Muscle
>> >
>> > Hi Kids!
>> >
>> > I'm going to be doing some work with muscle(frozen sections)  pretty
>> soon.
>> > I've always heard horror stories about freeze artifact and things like
>> > that. I just wanted to know if there is any good reference out there
>> that
>> > so I can read up on muscle techniques.
>> >
>> > Thanks,
>> > Margaret
>> >
>> >
>>
>>
>>
>>
>>
>>
>> ______________________________________________________
>> Get Your Private, Free Email at http://www.hotmail.com
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 21:03:13 -0600
>From: dave@biocare.net
>Subject: Steamer and CAP regulation
>
>I believe their must be a misunderstanding.  There are many papers
>validating the steam method.  Unless there has been a big pay-off (this
>is a joke), I do not believe this is a new CAP regulation.
>Immunohistochemistry markers are considered a Class 1 procedure and are
>not regulated by FDA.  Whether you use a waterbath or steamer, or
>pressure cooker or microwave to heat tissue, there should be very little
>difference in the results, if applied correctly.  We all have to
>validate our procedures with negative and positive controls that is
>signed off by a pathologist.  Now if a procedure is approved by the FDA,
>that is a different story.
>
>I personally talked to Dako, and they said they know nothing about a CAP
>ruling on steamer.
>
>David Tacha
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 21:04:33 -0600
>From: "D. Hammer" <hammerd@u.washington.edu>
>Subject: Re: double labeling -Reply
>
>Hey Karen,
>
>Good to see you on the histonet :)  I will book your address and write.
>
>Don
>
>
>
>
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Don Hammer, Administrative Director            UNIVERSITY OF WASHINGTON
>Hospital Pathology, Box 356100                     MEDICAL CENTER
>1995 NE Pacific St.
>Seattle Washington, 98195                  ~Where Knowledge Comes To Life~
>(206) 548-6401 Fax: (206) 548-4928
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>
>On Tue, 27 Oct 1998, Karen K. Dulany wrote:
>
>> Dear Nora, We do double staining a lot, and have even gone on to do
>> triple and quadruple staining.  When you get 4 different antibodies
>> and 4 different colors on one slide, it is very beautiful.  Time
>> consuming but very pretty when all are positive for the antibodies
>> you hope to see.  If I can be of further help, I will try to help
>> you.
>> Karen Dulany HTL (ASCP)
>> Univ. of NE Medical Center
>> Eppley Instiute for Cancer Research
>> Omaha, NE
>> 402-559-5123
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 21:23:10 -0600
>From: larisonk@UONEURO.uoregon.edu
>Subject: double-labeling
>
>Histonetters,
>
>There's a nice journal article on double-labeling two monoclonals of like
>isotype or two primaries from the same species using fluorescent tyramides.
>I'm finding that it works quite well with the red or the Cy-3 tyramide and
a
>fluorescein secondary.  If you're interetested, see J Histochem Cytochem
44,
>1331, 1996.
>
>Karen Larison
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 21:23:40 -0600
>From: ga2clowns@juno.com (Billie L. Swisher)
>Subject:
>
>
>___________________________________________________________________
>You don't need to buy Internet access to use free Internet e-mail.
>Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
>or call Juno at (800) 654-JUNO [654-5866]
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 21:24:13 -0600
>From: "P. Emry" <emry@u.washington.edu>
>Subject: Re: Sliding microtome
>
>Lynn,
>Amazing!  I am just trying to do that and was hoping I could get someone
>in to teach me...Anyone in the Seattle area?  I would happily leave the
>lab door unlocked if you promised to come and steal my sliding micrtome.
>This is hard.
>Trisha
>
>
>On Tue, 27 Oct 1998, Lynn Gardner wrote:
>
>> To all concerned,
>>
>> Does anyone have or know of anyone who has a sliding microtome they are
>> wanting to get rid of or anyone who would cut tissue sections of breast
>> tissue on a sliding microtome? Need the answers as soon as possible.
>>
>> Thanks all for your help!
>> Lynn Gardner
>>
>>
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 23:15:05 -0600
>From: Judith McNaughtan <j.mcnaughtan@dent.unimelb.edu.au>
>Subject: Re: double labeling
>
>At 15:45 27/10/98 -0700, vector@vectorlabs.com wrote:
>>For those interested in double (or triple) immunolabeling of antigens on
>>the same tissue section, using a combination of peroxidase and/or
>>alkaline phosphatase reagents, Vector Laboratories features a section
>>about this on our website. There are about 20 color photomicrograph
>>examples, some general considerations for this application, and a
>>protocol. This information was featured in a Vector newsletter alittle
>>while ago, but this is still available, and current, and will be mailed
>>to those who would like to receive their own copy. Please contact Vector
>>by phone, fax or e-mail to request a multiple antigen labeling Vectagram
>>copy.
>>
>>Hopefully this will answer alot of questions about the application.
>>
>>Sincerely,
>>
>>
>>Technical Services
>>
>>Vector Laboratories
>>30 Ingold Road
>>Burlingame, CA 94010
>>
>>Tel:  (650) 697-3600
>>Fax:  (650) 697-0339
>>E-mail:  vector@vectorlabs.com
>>Website:  http://www.vectorlabs.com
>>
>>
>>I would like a copy of your Vectagram on multiple antigen labelling.
>
>
>
>----------------------------------------------------------------------
>
>Date: 27 Oct 1998 23:15:26 -0600
>From: <johnmac@execulink.com>
>Subject: Microwave Pressure Cooker For HIER
>
> Has any one ever encountered nuclear bubbling when using a microwave
pressure
>cooker for HIER?  Have I just overcooked them??
>
>John
>
>
>
>----------------------------------------------------------------------
>
>Date: 28 Oct 1998 00:59:13 -0600
>From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
>Subject: Re: Steamers for IHC HIER
>
>On Tue, 27 Oct 1998, Sarah A. Jones wrote:
>>                                                        ... The rep
>> called us yesterday and told us that the CAP had just issued a new
>> regulation stating that steamers could no longer be used for heat
>> induced epitope retreival (HIER).
>>              .... getting the information
>> for us, as well as the substitute method; but I have yet to see any
>> documentation.
>
> Some salesman, overly greedy for his commission, is trying to
> frighten you into buying something expensive. If he claims to
> be from a reputable company, call their free 800 number and
> ask to speak to someone in authority, or send a snail mail
> letter to the Managing Director, and a copy to somebody senior
> in your own institution. Do not be intimidated. Heat is heat
> and in the presence of water it will "retrieve epitopes"
> however it is applied, subject to certain antigen-dependent
> idiosyncrasies of pH etc.
>
> If something as simple as a rice steamer or pressure cooker
> is adequate for the work you are doing, go on using it.
> When you are asked to do things that need more expensive
> equipment, ask your boss if it's really needed, and then
> invite several suppliers to give quotations. Tell them all
> that they are competing with their rivals. If you can get the
> same thing from a non-laboratory source it may well be much
> cheaper. Remember all those $1000 lavatory seats sold to
> the U.S. army?
>
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON,  Canada  N6A 5C1
>   E-mail: kiernan@uwo.ca
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 28 Oct 1998 00:59:38 -0600
>From: "Mick Rentsch" <ausbio@nex.com.au>
>Subject: Re: Microwave Pressure Cooker For HIER
>
>dear John,
>while I have'nt seen this artifact using pressure cookers I have seen it in
>Microwave recovery when I've overzapped'em, I strongly suspect it may be
>protein disruption caused by sudden release of pressure with the contents
>still at 121C. Rapid exhausting of autoclaves or pressure cookers is not
>recommended, suggest you at least wait for the pressure gauge to read zero
>or for the safety button to drop before you open the lid.
>Regards Mike Rentsch (Downunder)
>- -----Original Message-----
>From: John & Tracey MacKinnon <johnmac@execulink.com>
>To: 'histonet@Pathology.swmed.edu' <histonet@Pathology.swmed.edu>
>Date: Wednesday, 28 October 1998 4:30
>Subject: Microwave Pressure Cooker For HIER
>
>
>> Has any one ever encountered nuclear bubbling when using a microwave
>pressure cooker for HIER?  Have I just overcooked them??
>>
>>John
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 28 Oct 1998 01:00:00 -0600
>From: kevin gibbon <gibbowax@uniserve.com>
>Subject: Digital camera
>
>Hi everyone,
>I would like to ask if anyone has bought a digital camera for their
>microscope recently, I would like to hear their comments and info about
>price, adapters etc.
>
>Vendors, please feel welcome to reply as well :)
>
>Kevin Gibbon
>Wax-it
>Histology Services
>
>
>
>Here are the messages received yesterday!
>




<< Previous Message | Next Message >>