Re: Staining Silicone

<< Previous Message | Next Message >>
From:John Difford <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Attn Lee Hadley

Re: Staining Silicone in tissue

Dear Lee,

The difficulty with demonstrating silicone in tissue is that it can be
confused with normal tissue lipid.

One approach to the problem is as follows: Cut a frozen section of the
tissue, stain the the tissue lipid black with osmium tetroxide, the
silicone will not react with this and can be demonstrated using an Oil Red
O technique on top.

I have done this, many years ago, but I do not have a set protocol or
reference it. Its a case of make-it- up-a- you-go on this one.


John Difford
Histopathology Dept
Royal Free Hospital
London, England

<< Previous Message | Next Message >>