Re: Staining Silicone

<< Previous Message | Next Message >>
From:Joachim Siegmund <jsiegmu@ibm.net> (by way of histonet)
To:histonet <histonet@magicnet.net>
Reply-To:
Content-Type:text/enriched; charset="us-ascii"

 John Difford wrote: 

<paraindent><param>left</param>

Attn Lee Hadley

Re: Staining Silicone in tissue

Dear Lee,

The difficulty with demonstrating silicone in tissue is that it can be
confused with normal tissue lipid.

One approach to the problem is as follows: Cut a frozen section of the
tissue, stain the the tissue lipid black with osmium tetroxide, the
silicone will not react with this and can be demonstrated using an Oil
Red O technique on top.

I have done this, many years ago, but I do not have a set protocol or
reference it. Its a case of make-it- up-a- you-go on this one.

Regards

John Difford Histopathology Dept Royal Free Hospital London, England

</paraindent>

   Dear Histonetters

I think that it is not state of the art to use osmium tetroxide today
unless it's absoloutly necessary. I found a wonderful working method on
<bold><<http://www.afip.il/wolov/oro1.html>
http://www.afip.il/wolov/oro1.html </bold> by F.B.Johnson, M.D. It's an
Oil Red in Propylene Glycol method and works fine also with Sudan dyes.
Mr Johnson recommends staining in Oil Red from 24 h to 5 days. My
experience is that it's long enough to stain overnight.
<bold>Results:</bold> poly-ethelene, polymethyl methacrylate and
silicone    --cherry red Nuclei
------------------------------------blue

Regards

Joachim Siegmund BTA Hamburg, Germany    




<< Previous Message | Next Message >>