Re: Pepsin

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From:brian chelack <> (by way of histonet)
To:histonet <>
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Hello Cindy;
We have been running FactorVIII for the last few years and use Protease XIV
@ 0.5
mg/ml in PBS with good results.  Our antibody is purchased from Dako.  I
would try
your usual protease method first as I suspect the stain is fairly robust,
most of the
Pepsin digestion pretreatments I have read about in the past also work just
using other enzymatic protocols.


BJC - Brian		PS -  I haven't forgotten about the Rota....

On Thu, 29 Oct 1998 09:42:10 +0600 Cindy Chard-Bergstrom wrote:

> From: Cindy Chard-Bergstrom <>
> Date: Thu, 29 Oct 1998 09:42:10 +0600
> Subject: Pepsin
> To:
> Hello Everyone,
>     Has anyone out there had any experience using Pepsin as
> pretreatment for IHC's. If so what is the concentration that you have
> used. I am using factor VIII antibody and pepsin pretreatment is
> recommended. I have pepsin on had but it is not from the company that
> makes the primary antibody. Any advice is welcome.
> Cindy Chard-Bergstrom

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