Re: Fwd: Alternatives to Celloidin

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From:"J. A. Kiernan" <> (by way of histonet)
To:histonet <>
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On Mon, 26 Oct 1998, Steven E. Slap wrote:

> I received this question today, and don't know the answer.
> I have discovered a method for the removal of a single layer of cells
> by attachment to Celloidin. The celloidin is applied as a fluid and
> allowed to dry ...
>                          ... I wonder if there is
> any alternative to celloidin that is water soluble and requires no use
> of organic solvents.

   A colleague of mine (now retired) stripped endothelial cells
   from the opened aorta (rat, rabbit) by pressing against a
   gelatin-coated slide and then pealing off. I never watched him
   do it, and have no technical details. It does suggest,
   however, that gelatin might be sticky enough for your

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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