Re: Fwd: Alternatives to Celloidin
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Mon, 26 Oct 1998, Steven E. Slap wrote:
> I received this question today, and don't know the answer.
> I have discovered a method for the removal of a single layer of cells
> by attachment to Celloidin. The celloidin is applied as a fluid and
> allowed to dry ...
> ... I wonder if there is
> any alternative to celloidin that is water soluble and requires no use
> of organic solvents.
A colleague of mine (now retired) stripped endothelial cells
from the opened aorta (rat, rabbit) by pressing against a
gelatin-coated slide and then pealing off. I never watched him
do it, and have no technical details. It does suggest,
however, that gelatin might be sticky enough for your
purposes.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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