Re: Double Staining

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From:Dako Corporation <> (by way of histonet)
To:histonet <>
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DAKO offers a multistain IHC system based on a non-avidin biotin technology.
Any combination of polyclonal and monoclonal antibodies can be used. The kit
provides DAB and Fast Red as the chromogens.

The technique takes a different approach. After building up the staining
complex for a particular antigen (e.g. p53 with DAB), a reagent is used to
strip off the immunological complex from the specimen but the chromogen
precipitate is left behind. You can then pursue the next antigen of interest
with a different chromogen and repeat the process. This eliminates the cross
reactivity between the second (or third or fourth) antibody with the first

Results are available in just over an hour for doublestaining.

We presented this technique at the US and Canadian Academy of Pathology and
the manuscript (with 4 color pictures )is available. In this study, we have
described a method for multi-staining (double, triple and quadruple) of
co-localized antigens in paraffin-embedded, formalin-fixed tissues and blood
smears. To get a copy, contact DAKO at 800/424-0021 or drop us an e-mail: It needs to be mailed so do leave your mailing
address. Ask for literature number: PR0074 0297 38009.

Christopher Ung
Director, Product Management
DAKO Corporation

-----Original Message-----
From: Nora Fox [mailto:nefox@leland.Stanford.EDU]
Sent: Tuesday, October 27, 1998 11:47 AM
Subject: Re: double labeling

I would like to see this too--as may lots of others.

Thank you to all of you who have responded to my many questions.


>>Good Morning,
>>        Would someone who has some experience with double labeling[IHC]
>>please e-mail me directly? I have a few questions from one of my
>>colleagues...we're interested in one Ab w/DAB and one with alk.phos.
>>				Best Regards,
>>				Mary Vaughan HT(ASCP)
>>				Roswell Park Cancer Institute
>>				Pharmacology + Therapeutics
>>				Elm + Carlton Sts.  CDC-121
>>				Buffalo, NY 14263
Nora Fox
Division of Orthopaedic Surgery
Stanford University
Edwards R144, MC 5341
300 Pasteur Drive
Stanford, CA 94305
phone: (650)725-2746
fax:  (650)723-6396

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