Re: Cartilage Decalcification
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From: | Rebecca S Smith <bssvpisu@iastate.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
We find that soaking cartilage areas of the block with 5% ammonium
hydroxide does the trick!
At 08:53 AM 10/21/98 +0200, Pierre Mainil wrote:
>Hello histonetters,
>
>Another question to cartilage lovers.
>What is the best decalcification system.
>We observe important variability with Saf O staining although using
>cetylpyrimidium.
>Has some one ever used EDTA with etylpyrimidium during the
>decalcfication process.
>Advice and help appreciated.
>
>
>Pierre Bern
>
>
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