Re: Cartilage Decalcification

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From:Rebecca S Smith <> (by way of histonet)
To:histonet <>
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We find that soaking cartilage areas of the block with 5% ammonium
hydroxide does the trick!

At 08:53 AM 10/21/98 +0200, Pierre Mainil wrote:
>Hello histonetters,
>Another question to cartilage lovers.
>What is the best decalcification system.
>We observe important variability with Saf O staining although using
>Has some one ever used EDTA with etylpyrimidium  during the
>decalcfication process.
>Advice and help appreciated.
>Pierre Bern

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