Re: Cartilage Decalcification

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From:Rebecca S Smith <bssvpisu@iastate.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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We find that soaking cartilage areas of the block with 5% ammonium
hydroxide does the trick!



At 08:53 AM 10/21/98 +0200, Pierre Mainil wrote:
>Hello histonetters,
>
>Another question to cartilage lovers.
>What is the best decalcification system.
>We observe important variability with Saf O staining although using
>cetylpyrimidium.
>Has some one ever used EDTA with etylpyrimidium  during the
>decalcfication process.
>Advice and help appreciated.
>
>
>Pierre Bern
>
>




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