Re: Cartilage Decalcification

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From:Jerry Wilson <jwilso70@bellsouth.net> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Trisha, this is fairly common procedure in out lab.  we keep a small
container of  "roughly 5-10%" solution of am. hydroxide available at the
microtomes for sectioning of hard, dense, cartilaginous blocks.  after
facing the blocks (and ruining a couple of knives) we place the block in
the solution for a couple of minutes and try again.  "usually" we have good
results with the first few sections.  It's worth a try--let me know it it
works for you
jerry

P. Emry wrote:

> Hi Rebecca,
>
> I have not heard of the am. hydroxide method.  What does this do the the
> bone part of the specimen?  How long do you leave it in it?
>
> Thanks,
> Trisha
> U of Washington
>
> On Wed, 21 Oct 1998, Rebecca S Smith wrote:
>
> > We find that soaking cartilage areas of the block with 5% ammonium
> > hydroxide does the trick!
> >
> >
> >
> > At 08:53 AM 10/21/98 +0200, Pierre Mainil wrote:
> > >Hello histonetters,
> > >
> > >Another question to cartilage lovers.
> > >What is the best decalcification system.
> > >We observe important variability with Saf O staining although using
> > >cetylpyrimidium.
> > >Has some one ever used EDTA with etylpyrimidium  during the
> > >decalcfication process.
> > >Advice and help appreciated.
> > >
> > >
> > >Pierre Bern
> > >
> > >
> >
> >
> >




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