Hi Rebecca,

I have not heard of the am. hydroxide method.  What does this do the the
bone part of the specimen?  How long do you leave it in it?

Thanks,
Trisha
U of Washington



On Wed, 21 Oct 1998, Rebecca S Smith wrote:

> We find that soaking cartilage areas of the block with 5% ammonium
> hydroxide does the trick!
>
>
>
> At 08:53 AM 10/21/98 +0200, Pierre Mainil wrote:
> >Hello histonetters,
> >
> >Another question to cartilage lovers.
> >What is the best decalcification system.
> >We observe important variability with Saf O staining although using
> >cetylpyrimidium.
> >Has some one ever used EDTA with etylpyrimidium  during the
> >decalcfication process.
> >Advice and help appreciated.
> >
> >
> >Pierre Bern
> >
> >
>
>
>