Re: Cartilage Decalcification

<< Previous Message | Next Message >>
From:"P. Emry" <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Hi Jerry,
I use am. hydroxide to bleach skulls..under the hood.  How do your sinuses
hold up using it at almost nose level?  Is there a trick to this?
U of WA, Seattle

On Wed, 21 Oct 1998, Jerry Wilson wrote:

> Trisha, this is fairly common procedure in out lab.  we keep a small
> container of  "roughly 5-10%" solution of am. hydroxide available at the
> microtomes for sectioning of hard, dense, cartilaginous blocks.  after
> facing the blocks (and ruining a couple of knives) we place the block in
> the solution for a couple of minutes and try again.  "usually" we have good
> results with the first few sections.  It's worth a try--let me know it it
> works for you
> jerry
> P. Emry wrote:
> > Hi Rebecca,
> >
> > I have not heard of the am. hydroxide method.  What does this do the the
> > bone part of the specimen?  How long do you leave it in it?
> >
> > Thanks,
> > Trisha
> > U of Washington
> >

<< Previous Message | Next Message >>