Re: Cartilage Decalcification
<< Previous Message | Next Message >>
| From: | "P. Emry" <emry@u.washington.edu> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Hi Jerry,
I use am. hydroxide to bleach skulls..under the hood. How do your sinuses
hold up using it at almost nose level? Is there a trick to this?
Thanks,
Trisha
U of WA, Seattle
On Wed, 21 Oct 1998, Jerry Wilson wrote:
> Trisha, this is fairly common procedure in out lab. we keep a small
> container of "roughly 5-10%" solution of am. hydroxide available at the
> microtomes for sectioning of hard, dense, cartilaginous blocks. after
> facing the blocks (and ruining a couple of knives) we place the block in
> the solution for a couple of minutes and try again. "usually" we have good
> results with the first few sections. It's worth a try--let me know it it
> works for you
> jerry
>
> P. Emry wrote:
>
> > Hi Rebecca,
> >
> > I have not heard of the am. hydroxide method. What does this do the the
> > bone part of the specimen? How long do you leave it in it?
> >
> > Thanks,
> > Trisha
> > U of Washington
> >
<< Previous Message | Next Message >>