RE: what is "standard" pH for EDTA?

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA> (by way of histonet)
To:histonet <>
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I assume that you are refering to 1mM EDTA pH8.0, that is recommended by
some suppliers for some of their antibodies.
We also use EDTA at pH6.0 for other antibodies.



>From: 	Kathy Oprea[]
>Sent: 	October 19, 1998 3:21 PM
>Subject: 	what is "standard" pH for EDTA?
>This question has come up in our department and I did not have the
>answer- sooooo, guess who I thought of? Histonet!!  :-)  Does anyone
>out there know the standard pH for EDTA used for antigen retrieval?
>My experience is strictly with citrate buffer. Thank you all in
>advance. I will post answers I receive.
>Kathy Oprea
>************ Thank you ************
>* Kathy Oprea                     *
>* University of Georgia           *
>* College of Veterinary Medicine  *
>* Athens, GA 30602-7388           *
>*         *
>The worker who's never said "Thank
>God it's Friday," probably never
>really worked.

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