Kathy, we use it at a pH of 8 for all our retrieved antibodies except
HMB45 for which we still find that a citrate buffer works best.

Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

> -----Original Message-----
> From:	Kathy Oprea [SMTP:koprea@calc.vet.uga.edu]
> Sent:	Monday, October 19, 1998 3:21 PM
> To:	histonet@pathology.swmed.edu
> Subject:	what is "standard" pH for EDTA?
>
> This question has come up in our department and I did not have the
> answer- sooooo, guess who I thought of? Histonet!!  :-)  Does anyone
> out there know the standard pH for EDTA used for antigen retrieval?
> My experience is strictly with citrate buffer. Thank you all in
> advance. I will post answers I receive.
>
> Kathy Oprea
> ************ Thank you ************
> * Kathy Oprea                     *
> * University of Georgia           *
> * College of Veterinary Medicine  *
> * Athens, GA 30602-7388           *
> * KOPREA@calc.vet.uga.edu         *
> The worker who's never said "Thank
> God it's Friday," probably never
> really worked.
> ************************************