We buy it from Shandon.  They call it Cytospin Collection Fluid.  We call it
Saccomanno Fixative.  We use it to fix sputum samples.

> ----------
> From: 	Pat Stockton[SMTP:stockton@niehs.nih.gov]
> Sent: 	Wednesday, October 21, 1998 5:30 AM
> To: 	Histonet@pathology.swmed.edu
> Subject: 	saccomano's fixative
>
> Would love to hear from anyone who has used/heard of Saccomano's (sp?)
> fixative.
> Big thanks!
> pat
>
> >X-Sender: foley1@pop.niehs.nih.gov
> >Mime-Version: 1.0
> >Date: Mon, 19 Oct 1998 09:37:21 -0400
> >To: STOCKTON@niehs.nih.gov
> >From: Julie Foley <foley1@niehs.nih.gov>
> >Subject: saccomano's fixative
> >
> >Pat,
> >Can you do a posting on the histo site and see if you get any info on
> this
> >one for Rob?
> >Thanks
> >Julie
> >
> >
> >
> >>X-Sender: slebos@drucker.niehs.nih.gov (Unverified)
> >>Date: Sun, 18 Oct 1998 23:54:25 -0400
> >>To: Julie Foley <foley1@niehs.nih.gov>
> >>From: Robbert Slebos <slebos@niehs.nih.gov>
> >>Subject: saccomano's fixative
> >>Cc: slebos@niehs.nih.gov
> >>Mime-Version: 1.0
> >>
> >>Julie,
> >>
> >>Pardon my ignorance here, but I've looked and not found any decent
> recipe
> >>for what's called Saccomano's fixative. My main interest is in the
> presence
> >>of any cross-linkers that could mutilate our precious DNA <g>. It's for
> >>bronchial washings, so not too many enzymes or other gooey stuff
> expected.
> >>I was thinking of simply putting it in 10-20% ethanol.
> >>
> >>Thanks,
> >>Rob.
> >>
> >>----------------------------------------------------------------------
> >>Robbert J.C. Slebos, Ph.D.
> >>Visiting Scientist
> >>National Institute of Environmental Health Sciences
> >>Laboratory of Molecular Carcinogenesis
> >>Maildrop C2-01
> >>P.O.Box 12233
> >>Research Triangle Park, NC 27709
> >>
> >>Telephone: 919-541-2694
> >>Facsimile: 919-541-2511
> >>E-mail: slebos@niehs.nih.gov
> >>----------------------------------------------------------------------
> >>
> >
>
> Patricia Stockton, HT
> Stockton@niehs.nih.gov
>
>
>