Hi Nora,

I used the Vector kit once for this purpose because I thought I had made up
the substrate solution wrong on an IHC run. I happened to have some
intestinal sections already on slides so I made up new solution with the
kit and it was positive for endogenous alkaline phosphate in the
intestines. I have not used it any more extensively than that, but the
stain was plenty dark enough and unequivocal in that circumstance.

Cindy Farman

-----Original Message-----
From:	Nora Fox [SMTP:nefox@leland.Stanford.EDU]
Sent:	Tuesday, October 27, 1998 11:50 AM
To:	histonet@Pathology.swmed.edu
Subject:	Re: alk phos staining/kits

Thanks, Gayle.  Have you tried using the kit for this purpose?  Has anyone
else out there?

>Nora,
>Donna's suggestion for alk phos was excellent, and this is a what if
>question, or can you:  Why not use an alkaline phosphatase kit,
>say Vectors, to stain the cells?  so easy and inexpensive, and also
>is anyone doing this or tried it?
>This is the same kit used for alk phos immunostaining. All ingredients are
>present, ready to go.
>
>Gayle Callis

Nora Fox
Division of Orthopaedic Surgery
Stanford University
Edwards R144, MC 5341
300 Pasteur Drive
Stanford, CA 94305
phone: (650)725-2746
fax:  (650)723-6396