RE: Perfusion Setup

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From:Cynthia Favara <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

	I work in a fume hood. Use a large metal pan in which I place a
styrofoam block, the kind that 25ml centrifuge tubes come in, covered with
foil in the center of the pan. I use bench paper on the block to absorb
fluids. Perfuse disect and then wrap the ramains in the bench paper for
disposal. Excess liquid is collected in the pan and disposed per our
protocol. Works well for me hope this helps.
Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286

> ----------
> From:[]
> Sent: 	Monday, October 19, 1998 1:45 PM
> To:
> Subject: 	Perfusion Setup
> Date:  10/19/1998  02:37 pm  (Monday)
> From:  Clover Daley
> To:  PCU_dom.api:""
> Subject:  Perfusion Setup
> In the near future we will be perfusing rats with
> Glutaraldehyde, Paraformaldehyde in Sodium Cacodylate
> buffer.
> I need to set up a well ventilated area in which we can work
> and also have a way to collect the run off of the chemicals.
> If anyone else is perfusing please tell me what you have
> found to work. It can be a purchased unit or something that
> has been self designed.
> Thank you
> Clover Daley
> Palmer Center for Chiropractic Research
> Davenport, IA 52803

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