RE: Neg Immunocontrols

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From:"Tarpley, John" <jtarpley@amgen.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Brian,
We've also done the "competition" type of experiments and sometimes they can
be very useful, but at other times the results can be confusing. I find them
most useful for checking cross reactivity with other family members of the
same antigen type.
John

> ----------
> From: 	brian chelack[SMTP:chelack@admin3.usask.ca]
> Sent: 	Monday, October 19, 1998 8:32 AM
> To: 	Tarpley, John
> Cc: 	A. Mark Briones; HistoNet Server
> Subject: 	RE: Neg Immunocontrols
>
> In my mind the best true control for a polyclonal antibody is the very
> same serum
> with the addition of the specific antigen it is directed against.




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