RE: Muscle freezing: Temperature

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From:"Tarpley, John" <> (by way of histonet)
To:histonet <>
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When I've done animal muscles I've sometimes found the commercially
available muscle clamps made for human samples are inadequate because they
either can't grip far enough into the thickness of the muscle or we wanted
to take a longer sample than the clamp was wide. To solve these problems we
made our own clamps by using a metal bar with a length the same as the
desired length of the sample with a hemostat welded to each end of the bar.
We could then clamp the muscle, cut on the outside of the clamp and either
allow the muscle to rest in the refrigerator or place the muscle (clamp and
all) directly into fixative as our protocol and the pathologist doing the
work required.

John Tarpley 15-2-B
Specialist Image Analysis & Immunohistochemistry
Amgen Inc
One Amgen Center Drive
Thousand Oaks, CA  91320

Views expressed are mine alone and do not represent the views of my employer

> ----------
> From: 	Tim Morken[]
> Sent: 	Thursday, October 29, 1998 5:25 AM
> To:
> Cc:
> Subject: 	RE: Muscle freezing: Temperature
> Vicki,
> You are seeing contraction in the muscle. The trick is to hold the
> muscle in a streched state until it cannot contract anymore.
	--snip snip--
> Tim Morken, B.S., EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology
> Centers for Disease Control
> MS-G32
> 1600 Clifton Rd.
> Atlanta, GA 30333

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