RE: Lead

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Fernando:

Choice method for the demonstration of lead in tissue and decalcified bone
is the Rhodizonate method. Using sodium rhodizonate as a chelating agent to
form a red complex.

Sections: Paraffin cut at 5-6 microns

Solutions:
Sodium rhodizonate		200 mg
DH20					99 cm
Glacial acetic acid		1 cm

Method:
1.	Sections to water.
2.	Transfer to rhodizonate solution for 1 hour.
3.	Wash in tap water.
4.	Counterstain in 0.1% light green in 1% acetic acid for 2 minutes.
5.	Rinse briefly in tap water.
6.	Mount in glycerin.

Results:
Lead salts-red
Background tissue-green

Reference:
Bancroft & Stevens (1977)Theory and Practice of Histological
Techniques,pp.205-206.

Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
	----------
	From:  Fernando Capela e Silva [SMTP:fcs@uevora.pt]
	Sent:  Friday, October 16, 1998 8:46 AM
	To:  histonet@Pathology.swmed.edu
	Subject:  Lead

	I am looking for a method to demonstrate lead in bone and kidney.

	Thanks in advance for your help.

	Fernando Capela e Silva
	Departamento de Biologia
	Universidade de Evora
	Apartado 94
	7001 Evora Codex
	PORTUGAL

	Telephone: +351-66-740 98 81
	Telefax: +351-66-711 231
	E-mail: fcs@uevora.pt
	http://home.dbio.uevora.pt/~fcs/FCAPELAeSILVA.html





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