RE: Lead
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From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
Fernando:
Choice method for the demonstration of lead in tissue and decalcified bone
is the Rhodizonate method. Using sodium rhodizonate as a chelating agent to
form a red complex.
Sections: Paraffin cut at 5-6 microns
Solutions:
Sodium rhodizonate 200 mg
DH20 99 cm
Glacial acetic acid 1 cm
Method:
1. Sections to water.
2. Transfer to rhodizonate solution for 1 hour.
3. Wash in tap water.
4. Counterstain in 0.1% light green in 1% acetic acid for 2 minutes.
5. Rinse briefly in tap water.
6. Mount in glycerin.
Results:
Lead salts-red
Background tissue-green
Reference:
Bancroft & Stevens (1977)Theory and Practice of Histological
Techniques,pp.205-206.
Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
----------
From: Fernando Capela e Silva [SMTP:fcs@uevora.pt]
Sent: Friday, October 16, 1998 8:46 AM
To: histonet@Pathology.swmed.edu
Subject: Lead
I am looking for a method to demonstrate lead in bone and kidney.
Thanks in advance for your help.
Fernando Capela e Silva
Departamento de Biologia
Universidade de Evora
Apartado 94
7001 Evora Codex
PORTUGAL
Telephone: +351-66-740 98 81
Telefax: +351-66-711 231
E-mail: fcs@uevora.pt
http://home.dbio.uevora.pt/~fcs/FCAPELAeSILVA.html
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