RE: ED-1-staining problem
<< Previous Message | Next Message >>
| From: | "Baileyhealy, Irene {USA~Palo Alto}" <IRENE.BAILEYHEALY@roche.com> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Hi Kelly
I'm using the Serotec ED-1 Ab @ 1:250 for an hour @ RT, including a
citrate/microwave antigen retrieval, 2* Ab is from Vector - biotinylated
horse anti mouse IgG, rat adsorbed @ 1:200 and the ABC-AP detection method.
What staining protocol do you use?
Irene
Palo Alto
CA
-----Original Message-----
From: Kelly Randall [SMTP:kellyr@neuro.hfh.edu]
Sent: Tuesday, October 20, 1998 12:03 PM
To: IRENE.BAILEYHEALY@Roche.COM
Cc: "'histonet@pathology.swmed.edu'"@neuro.hfh.edu
Subject: Re: ED-1-staining problem
What type of staining protocol are you using?
We stain rat brain with Serotec ED-1 antiboday and get great
staining.
Kelly Randall
<< Previous Message | Next Message >>