Karen:

Dent's cold fixative is used for some fluorescence labeling techniques:

1 part (DMSO) dimethyl sulfoxide
4 parts methanol

Stored in the freezer at -20 degree centigrade for Blastogenetics, or in the
cryostat on frozen sections for Immunofluorescence.

Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center

	----------
	From:  Karen D. Larison [SMTP:LARISONK@UONEURO.uoregon.edu]
	Sent:  Monday, October 26, 1998 6:22 PM
	To:  HistoNet@Pathology.swmed.edu
	Subject:  Dent's fixative

	Histonetters,

	I've been asked about Dent's fixative.  Does anyone know what this
is?

	Thanks a bunch.  -Karen