Fixation of bones

<< Previous Message | Next Message >>
From:Roger Gallant <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Hello histonetters,

This is a question for the "boneheads". Has anyone every experienced
more difficulty cutting MMA embedded bone fixed in 10% formalin? We
normally fix in 70% and have never experienced the shredding and tearing
of  rat bones(tibias and femurs) that is occuring with these rat bones
fixed in formalin. Our cycle etc.. is otherwise the same, and we were
wondering if the fixation could have an impact? Or perhaps the length of
time in the fixative? (2-3 months) Any ideas?

C. Washer
Senneville, Quebec

<< Previous Message | Next Message >>