Collagen gels
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From: | Mary Vaughan <mvaughan@sc3102.med.buffalo.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear Margaret,
>snip<
> Has anyone any experience of immuno staining cells cultured
> in collagen gels? I have been trying to stain paraffin
> processed gels for some time with very mixed results, mostly
> the antibodies just stick to the gel, despite blocking.
I've done quite a bit of staining on paraffin sections of cells
and/or organoids in collagen gel. It seems to depend on the antibody. Did
you try a lower Primary Ab concentration [with perhaps an overnight
incubation]? How do your IgG matched negative controls look?
Best Regards,
Mary Vaughan HT(ASCP)
Roswell Park Cancer Institute
Pharmacology + Therapeutics
Elm + Carlton Sts. CDC-121
Buffalo, NY 14263
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