Collagen gels

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From:Mary Vaughan <> (by way of histonet)
To:histonet <>
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Dear Margaret,

>          Has anyone any experience of immuno staining cells cultured
>          in collagen gels? I have been trying to stain paraffin
>          processed gels for some time with very mixed results, mostly
>          the antibodies just stick to the gel, despite blocking.

        I've done quite a bit of staining on paraffin sections of cells
and/or organoids in collagen gel. It seems to depend on the antibody. Did
you try a lower Primary Ab concentration [with perhaps an overnight
incubation]? How do your IgG matched negative controls look?
				Best Regards,
				Mary Vaughan HT(ASCP)
				Roswell Park Cancer Institute
				Pharmacology + Therapeutics
				Elm + Carlton Sts.  CDC-121
				Buffalo, NY 14263

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