Cartilage slides go for a swim...... -Reply

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From:Sarah Christo <schristo@CVM.TAMU.EDU> (by way of histonet)
To:histonet <>
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Dear Pierre,
  Have you tried celloidinizing the sections
after deparaffinization?  Sarah, Texas A&M

Pierre wrote:
Hello histonetters,

This is a question for the "cartilage fricks". Has anyone every
experienced difficulty with cartilage samples.?
After cutting of decalcified specimen (EdTA), the cut never stay on the
slides but always "Swim" during staining process. This problems does not
occur with all samples but more frequently with young cartilage donors.
This is probably due to the fact that they are highly negatively
Does histonetters have a possible solution.

Pierre Mainil
normally fix in 70% and have never experienced the shredding and tearing
of  rat bones(tibias and femurs) that is occuring with these rat bones
fixed in formalin. Our cycle etc.. is otherwise the same, and we were
wondering if the fixation could have an impact? Or perhaps the length of
time in the fixative? (2-3 months) Any ideas?

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