negative controls
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| From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
We often use the IgG isotype matched negative control, rather
than the primary host serum. Had a publication refused once when we did
the serum method, had to redo all the work, a tedious situation. In this
case will sometimes end up with a negative control for EACH antibody if the
isotypes are different.
Gayle Callis
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