negative controls

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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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We often use the IgG isotype matched negative control, rather
than the primary host serum.  Had a publication refused once when we did
the serum method, had to redo all the work, a tedious situation.   In this
case will sometimes end up with a negative control for EACH antibody if the
isotypes are different.

Gayle Callis

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