cryosectioning bony/fibrous tissue

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From:Nora Fox <nefox@leland.Stanford.EDU> (by way of histonet)
To:histonet <>
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Hello all!

My past experience has been cutting extremely soft tissue.  Can any of you
suggest a protocol for cutting semi-bony, fibrous tissue.  Specifically,
what temperature works well?  Do you have any other hints?  I am working
with snap frozen, unfixed tissue.


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