alizarin red for calcium

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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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Try Dahls' method from the AFIP manual, old green book, and make sure
the pH is adjusted to 6.36-6.4 with 0.1% ammonium hydroxide (10 mls of this
to 100 mls 1% alizarin red in distilled water.  It is best to fix with
an alcoholic fixation (alcohol formalin, avoid acid additives, the old
d'cal problem).  Carson's book has this method, but the pH I used above
seemed to work better but she gives some excellent hints and other
notes that may be important.  She has some differences in the recipe for
making up the dye solution.

Von Kossa's is a classic method, and simple to perform, you can counterstain
nicely with this method (black deposits show up with sharp contrast).
Alizarin Red gave some elegant results, however!

Good luck,

Gayle Callis

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