Dear Dr. Krivolapov, My I ask you the identity of the detergent and what is
the ratio added IPA for paraffin embedding?
Jeff Silverman
Southampton Hospital NY USA
peptolab@hamptons.com

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> From: Dr Y Krivolapov, MD <krivolapov@bfpg.ru>
> To: HistoNet Server <HistoNet@Pathology.swmed.edu>
> Subject: xylene AND alcohol substitude
> Date: Saturday, October 03, 1998 3:42 PM
>
>
> >> Is there a product that replaces xylene AND alcohols in the staining
> >> procedure?  Can you use it before and after the actual staining is
done?
> >>
> >> Thanks,
> >>
> >> Trisha
>
> Dear Trisha,
>
> We use 99% isopropyl alcohol (IPA) instead ethanol AND xylene AFTER
> staining. It is especially useful after staining of lymph nodes with
> Maximov-Giemsa method. My modification of this method is to use eosin and
> erytrosin mixture for differentiation overstained sections. We place a
drop
> of EE mixture over section moisten with a few drops of IPA. My laboratory
> use this modification more then 5 years and I never seen the same
excellent
> result in comparison with atlases of lymph nodes biopsy. And more, we use
> IPA with addition of small amount of detergent for dehydration of
samples. 4
> changes of 99% IPA+detergent - it is all of what you need between water
and
> paraffin. We never have had problems with any tissues (large samples of
skin
> or other the same difficult for embedding). Our HTs adore IPA.
>
> Dr Yuri Krivolapov MD
> Laboratory of Molecular Pathology and Cytogenetics
> Department of Haematology and Clinical Immunology
> Military Medical Academy
> St.-Petersburg, Russia
> krivolapov@bfpg.ru
>
>
>