Re: xylene AND alcohol substitude

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From:Jeff Silverman <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Dear Dr. Krivolapov, My I ask you the identity of the detergent and what is
the ratio added IPA for paraffin embedding?
Jeff Silverman
Southampton Hospital NY USA

> From: Dr Y Krivolapov, MD <>
> To: HistoNet Server <>
> Subject: xylene AND alcohol substitude
> Date: Saturday, October 03, 1998 3:42 PM
> >> Is there a product that replaces xylene AND alcohols in the staining
> >> procedure?  Can you use it before and after the actual staining is
> >>
> >> Thanks,
> >>
> >> Trisha
> Dear Trisha,
> We use 99% isopropyl alcohol (IPA) instead ethanol AND xylene AFTER
> staining. It is especially useful after staining of lymph nodes with
> Maximov-Giemsa method. My modification of this method is to use eosin and
> erytrosin mixture for differentiation overstained sections. We place a
> of EE mixture over section moisten with a few drops of IPA. My laboratory
> use this modification more then 5 years and I never seen the same
> result in comparison with atlases of lymph nodes biopsy. And more, we use
> IPA with addition of small amount of detergent for dehydration of
samples. 4
> changes of 99% IPA+detergent - it is all of what you need between water
> paraffin. We never have had problems with any tissues (large samples of
> or other the same difficult for embedding). Our HTs adore IPA.
> Dr Yuri Krivolapov MD
> Laboratory of Molecular Pathology and Cytogenetics
> Department of Haematology and Clinical Immunology
> Military Medical Academy
> St.-Petersburg, Russia

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