I'm not sure I understand this. Are you saying you use isopropyl alcohol
and detergent to deparaffinize sections? If so, could you give a
detailed procedure?

Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043




----Original Message Follows----
Date: Sat, 03 Oct 1998 22:42:07 +0300
From: "Dr Y Krivolapov, MD" <krivolapov@bfpg.ru>
Subject: xylene AND alcohol substitude
To: HistoNet Server <HistoNet@Pathology.swmed.edu>


>> Is there a product that replaces xylene AND alcohols in the staining
>> procedure?  Can you use it before and after the actual staining is
done?
>>
>> Thanks,
>>
>> Trisha

Dear Trisha,

We use 99% isopropyl alcohol (IPA) instead ethanol AND xylene AFTER
staining. It is especially useful after staining of lymph nodes with
Maximov-Giemsa method. My modification of this method is to use eosin
and
erytrosin mixture for differentiation overstained sections. We place a
drop
of EE mixture over section moisten with a few drops of IPA. My
laboratory
use this modification more then 5 years and I never seen the same
excellent
result in comparison with atlases of lymph nodes biopsy. And more, we
use
IPA with addition of small amount of detergent for dehydration of
samples. 4
changes of 99% IPA+detergent - it is all of what you need between water
and
paraffin. We never have had problems with any tissues (large samples of
skin
or other the same difficult for embedding). Our HTs adore IPA.

Dr Yuri Krivolapov MD
Laboratory of Molecular Pathology and Cytogenetics
Department of Haematology and Clinical Immunology
Military Medical Academy
St.-Petersburg, Russia
krivolapov@bfpg.ru









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